Product: NXPH3 Antibody
Catalog: DF4231
Description: Rabbit polyclonal antibody to NXPH3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 30 KD; 28kD(Calculated).
Uniprot: O95157
RRID: AB_2836582

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NXPH3 Antibody detects endogenous levels of total NXPH3.
RRID:
AB_2836582
Cite Format: Affinity Biosciences Cat# DF4231, RRID:AB_2836582.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA1159; Neurexophilin-3; NPH3; Nxph3; NXPH3_HUMAN; UNQ687/PRO1327;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O95157 NXPH3_HUMAN:

Highest level in brain.

Sequence:
MQLTRCCFVFLVQGSLYLVICGQDDGPPGSEDPERDDHEGQPRPRVPRKRGHISPKSRPMANSTLLGLLAPPGEAWGILGQPPNRPNHSPPPSAKVKKIFGWGDFYSNIKTVALNLLVTGKIVDHGNGTFSVHFQHNATGQGNISISLVPPSKAVEFHQEQQIFIEAKASKIFNCRMEWEKVERGRRTSLCTHDPAKICSRDHAQSSATWSCSQPFKVVCVYIAFYSTDYRLVQKVCPDYNYHSDTPYYPSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
45
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95157 As Substrate

Site PTM Type Enzyme
K98 Ubiquitination
Y106 Phosphorylation
S107 Phosphorylation
S200 Phosphorylation
S206 Phosphorylation
T209 Phosphorylation
Y242 Phosphorylation
S251 Phosphorylation

Research Backgrounds

Function:

May be signaling molecules that resemble neuropeptides. Ligand for alpha-neurexins (By similarity).

PTMs:

May be proteolytically processed at the boundary between the N-terminal non-conserved and the central conserved domain in neuron-like cells.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest level in brain.

Family&Domains:

Belongs to the neurexophilin family.

Restrictive clause

 

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For Research Use Only.
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