NLE1 Antibody - #DF4246
| Product: | NLE1 Antibody |
| Catalog: | DF4246 |
| Description: | Rabbit polyclonal antibody to NLE1 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 48 KD; 53kD(Calculated). |
| Uniprot: | Q9NVX2 |
| RRID: | AB_2836597 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4246, RRID:AB_2836597.
Fold/Unfold
AL022765; BC018399; FLJ10458; NLE 1; Nle; NLE1; NLE1_HUMAN; Notchless gene homolog; Notchless homolog 1 (Drosophila); Notchless homolog 1; Notchless protein homolog 1;
Immunogens
- Q9NVX2 NLE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAVPDEAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLPLAFFVHDAEIVSSLGKTLESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKGHRHWVLSISWSPDGRKLASGCKNGQILLWDPSTGKQVGRTLAGHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NVX2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot |
Research Backgrounds
Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).
Nucleus>Nucleolus.
Associates with the pre-60S ribosomal particle. Interacts (via WD repeats) with uL18 (By similarity). Interacts (via UBL domain) with MDN1 (via VWFA/MIDAS domain).
Belongs to the NLE1/RSA4 family.
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