NOLC1 Antibody | Affinity Biosciences

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IF/ICC

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NOLC1 Antibody - Western blot analysis of extracts from K562 cells, using NOLC1 antibody.

NOLC1 Antibody - Western blot analysis of extracts from K562 cells, using NOLC1 Antibody.

NOLC1 Antibody - DF4264 staining Hela cells by IF/ICC.

Product:	NOLC1 Antibody
Catalog:	DF4264
Description:	Rabbit polyclonal antibody to NOLC1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	73 KD; 74kD(Calculated).
Uniprot:	Q14978
RRID:	AB_2836615

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NOLC1 Antibody detects endogenous levels of total NOLC1.

RRID:

AB_2836615
Cite Format: Affinity Biosciences Cat# DF4264, RRID:AB_2836615.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

140 kDa nucleolar phosphoprotein; HCV NS5A trans regulated protein 13; HCV NS5A transactivated protein 13; HCV NS5A-transactivated protein 13; Hepatitis C virus NS5A transactivated protein 13; Hepatitis C virus NS5A-transactivated protein 13; KIAA0035; NOLC 1; NOLC1; NOLC1_HUMAN; NOPP 130; NOPP 140; NOPP130; Nopp140; NS5ATP13; Nucleolar 130 kDa protein; Nucleolar and coiled body phosphoprotein 1; Nucleolar and coiled body phosphprotein 1; Nucleolar and coiled-body phosphoprotein 1; Nucleolar phosphoprotein p130; Nucleolar protein p130; P130;

Immunogens

Immunogen:

Uniprot:

Q14978(NOLC1_HUMAN) >>Visit HPA database.

Gene(ID):

NOLC1(9221)

Sequence:

Q14978 NOLC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14978 As Substrate

Q14978

Site	PTM Type	Enzyme	Source
R7	Methylation		Uniprot
S28	Phosphorylation		Uniprot
K33	Acetylation		Uniprot
K33	Ubiquitination		Uniprot
K36	Ubiquitination		Uniprot
T38	Phosphorylation		Uniprot
T41	Phosphorylation		Uniprot
S55	Phosphorylation		Uniprot
K59	Ubiquitination		Uniprot
K67	Sumoylation		Uniprot
K76	Acetylation		Uniprot
S83	Phosphorylation		Uniprot
S84	Phosphorylation		Uniprot
S85	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
S90	Phosphorylation		Uniprot
S91	Phosphorylation		Uniprot
S124	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
S132	Phosphorylation		Uniprot
S173	Phosphorylation		Uniprot
S174	Phosphorylation		Uniprot
S175	Phosphorylation		Uniprot
T188	Phosphorylation		Uniprot
T191	Phosphorylation		Uniprot
K193	Sumoylation		Uniprot
K193	Ubiquitination		Uniprot
S216	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
S218	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
S220	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
S223	Phosphorylation		Uniprot
S224	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
T238	Phosphorylation		Uniprot
K251	Ubiquitination		Uniprot
S264	Phosphorylation		Uniprot
S265	Phosphorylation		Uniprot
S267	Phosphorylation		Uniprot
S270	Phosphorylation		Uniprot
S271	Phosphorylation		Uniprot
S272	Phosphorylation		Uniprot
K285	Ubiquitination		Uniprot
Y289	Phosphorylation		Uniprot
S290	Phosphorylation		Uniprot
S291	Phosphorylation		Uniprot
S296	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S332	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
K356	Acetylation		Uniprot
S361	Phosphorylation		Uniprot
S362	Phosphorylation		Uniprot
S363	Phosphorylation		Uniprot
S365	Phosphorylation		Uniprot
S366	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
T383	Phosphorylation		Uniprot
T384	Phosphorylation		Uniprot
K390	Acetylation		Uniprot
T395	Phosphorylation		Uniprot
S397	Phosphorylation		Uniprot
K401	Acetylation		Uniprot
K415	Acetylation		Uniprot
K415	Sumoylation		Uniprot
T438	Phosphorylation		Uniprot
K446	Acetylation		Uniprot
S456	Phosphorylation		Uniprot
S469	Phosphorylation		Uniprot
S470	Phosphorylation		Uniprot
S473	Phosphorylation		Uniprot
S476	Phosphorylation		Uniprot
S477	Phosphorylation		Uniprot
S504	Phosphorylation		Uniprot
K505	Acetylation		Uniprot
S508	Phosphorylation		Uniprot
K510	Acetylation		Uniprot
K511	Acetylation		Uniprot
S516	Phosphorylation		Uniprot
S517	Phosphorylation		Uniprot
S519	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
S521	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
S526	Phosphorylation		Uniprot
S538	Phosphorylation		Uniprot
S563	Phosphorylation		Uniprot
K572	Sumoylation		Uniprot
S578	Phosphorylation		Uniprot
K579	Acetylation		Uniprot
S580	Phosphorylation		Uniprot
S582	Phosphorylation		Uniprot
T597	Phosphorylation		Uniprot
K601	Acetylation		Uniprot
K604	Sumoylation		Uniprot
T607	Phosphorylation	P06493 (CDK1)	Uniprot
T610	Phosphorylation	P06493 (CDK1)	Uniprot
S622	Phosphorylation		Uniprot
S623	Phosphorylation	P17612 (PRKACA)	Uniprot
S637	Phosphorylation		Uniprot
R638	Methylation		Uniprot
S643	Phosphorylation		Uniprot
K647	Acetylation		Uniprot
K647	Ubiquitination		Uniprot
R648	Methylation		Uniprot
K663	Acetylation		Uniprot
K663	Methylation		Uniprot
K663	Sumoylation		Uniprot
K663	Ubiquitination		Uniprot
T665	Phosphorylation		Uniprot
S669	Phosphorylation		Uniprot
T675	Phosphorylation		Uniprot
S681	Phosphorylation		Uniprot
Y682	Phosphorylation		Uniprot
R683	Methylation		Uniprot
S686	Phosphorylation		Uniprot
S688	Phosphorylation		Uniprot
S693	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot

Research Backgrounds

Q14978_NOLC1_HUMAN

Function:

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.

PTMs:

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell.

Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm.
Note: Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.

Subunit Structure:

Heterodimer; heterodimerizes with TCOF1 following monoubiquitination. Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II. Interacts with DKC1/NAP57, NOP58 and fibrillarin (By similarity).

Family&Domains:

Belongs to the NOLC1 family.

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NOLC1 Antibody - #DF4264