NOL10 Antibody - #DF4265
(3)
Product: | NOL10 Antibody |
Catalog: | DF4265 |
Description: | Rabbit polyclonal antibody to NOL10 |
Application: | WB IHC IF/ICC |
Reactivity: | Human |
Prediction: | Pig, Horse, Rabbit, Dog |
Mol.Wt.: | 80 KD; 80kD(Calculated). |
Uniprot: | Q9BSC4 |
RRID: | AB_2836616 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Pig(80%), Horse(90%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
NOL10 Antibody detects endogenous levels of total NOL10.
RRID:
AB_2836616
Cite Format: Affinity Biosciences Cat# DF4265, RRID:AB_2836616.
Cite Format: Affinity Biosciences Cat# DF4265, RRID:AB_2836616.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ13938; FLJ14075; H_NH0074G24.1; NOL 10; Nol10; NOL10_HUMAN; Nucleolar protein 10; Polyglutamine binding protein 5; PQBP5;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9BSC4 NOL10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQVSSLNEVKIYSLSCGKSLPEWLSDRKKRALQKKDVDVRRRIELIQDFEMPTVCTTIKVSKDGQYILATGTYKPRVRCYDTYQLSLKFERCLDSEVVTFEILSDDYSKIVFLHNDRYIEFHSQSGFYYKTRIPKFGRDFSYHYPSCDLYFVGASSEVYRLNLEQGRYLNPLQTDAAENNVCDINSVHGLFATGTIEGRVECWDPRTRNRVGLLDCALNSVTADSEINSLPTISALKFNGALTMAVGTTTGQVLLYDLRSDKPLLVKDHQYGLPIKSVHFQDSLDLILSADSRIVKMWNKNSGKIFTSLEPEHDLNDVCLYPNSGMLLTANETPKMGIYYIPVLGPAPRWCSFLDNLTEELEENPESTVYDDYKFVTKKDLENLGLTHLIGSPFLRAYMHGFFMDIRLYHKVKLMVNPFAYEEYRKDKIRQKIEETRAQRVQLKKLPKVNKELALKLIEEEEEKQKSTWKKKVKSLPNILTDDRFKVMFENPDFQVDEESEEFRLLNPLVSKISEKRKKKLRLLEQQELREKEEEEEPEGKPSDAESSESSDDEKAWVEEVRKQRRLLQQEEKVKRQERLKEDQQTVLKPQFYEIKAGEEFRSFKDSATKQKLMNKTLEDRLKIEAKNGTLSVSDTTVGSKQLTFTLKRSEQQKKQQEAEKLHRQERKRLRRSAGHLKSRHKRGRSFH
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
90
Dog
90
Pig
80
Rabbit
80
Bovine
70
Chicken
70
Xenopus
60
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BSC4 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
K18 | Ubiquitination | Uniprot | |
S25 | Phosphorylation | Uniprot | |
K62 | Ubiquitination | Uniprot | |
K74 | Ubiquitination | Uniprot | |
S260 | Phosphorylation | Uniprot | |
K262 | Ubiquitination | Uniprot | |
K267 | Ubiquitination | Uniprot | |
K296 | Ubiquitination | Uniprot | |
K304 | Ubiquitination | Uniprot | |
Y339 | Phosphorylation | Uniprot | |
Y340 | Phosphorylation | Uniprot | |
S392 | Phosphorylation | Uniprot | |
K432 | Sumoylation | Uniprot | |
K451 | Ubiquitination | Uniprot | |
K456 | Ubiquitination | Uniprot | |
K464 | Ubiquitination | Uniprot | |
S475 | Phosphorylation | Uniprot | |
T481 | Phosphorylation | Uniprot | |
S500 | Phosphorylation | Uniprot | |
S511 | Phosphorylation | Uniprot | |
S514 | Phosphorylation | Uniprot | |
S543 | Phosphorylation | Uniprot | |
S547 | Phosphorylation | Uniprot | |
S548 | Phosphorylation | Uniprot | |
S550 | Phosphorylation | Uniprot | |
S551 | Phosphorylation | Uniprot | |
K589 | Ubiquitination | Uniprot | |
Y593 | Phosphorylation | Uniprot | |
K596 | Ubiquitination | Uniprot | |
K605 | Ubiquitination | Uniprot | |
S607 | Phosphorylation | Uniprot | |
S634 | Phosphorylation | Uniprot | |
S673 | Phosphorylation | Uniprot | |
S686 | Phosphorylation | Uniprot |
Research Backgrounds
Subcellular Location:
Nucleus>Nucleolus.
Family&Domains:
Belongs to the WD repeat NOL10/ENP2 family.
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