Product: NOL10 Antibody
Catalog: DF4265
Description: Rabbit polyclonal antibody to NOL10
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 80 KD; 80kD(Calculated).
Uniprot: Q9BSC4
RRID: AB_2836616

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Horse(90%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
NOL10 Antibody detects endogenous levels of total NOL10.
RRID:
AB_2836616
Cite Format: Affinity Biosciences Cat# DF4265, RRID:AB_2836616.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ13938; FLJ14075; H_NH0074G24.1; NOL 10; Nol10; NOL10_HUMAN; Nucleolar protein 10; Polyglutamine binding protein 5; PQBP5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MQVSSLNEVKIYSLSCGKSLPEWLSDRKKRALQKKDVDVRRRIELIQDFEMPTVCTTIKVSKDGQYILATGTYKPRVRCYDTYQLSLKFERCLDSEVVTFEILSDDYSKIVFLHNDRYIEFHSQSGFYYKTRIPKFGRDFSYHYPSCDLYFVGASSEVYRLNLEQGRYLNPLQTDAAENNVCDINSVHGLFATGTIEGRVECWDPRTRNRVGLLDCALNSVTADSEINSLPTISALKFNGALTMAVGTTTGQVLLYDLRSDKPLLVKDHQYGLPIKSVHFQDSLDLILSADSRIVKMWNKNSGKIFTSLEPEHDLNDVCLYPNSGMLLTANETPKMGIYYIPVLGPAPRWCSFLDNLTEELEENPESTVYDDYKFVTKKDLENLGLTHLIGSPFLRAYMHGFFMDIRLYHKVKLMVNPFAYEEYRKDKIRQKIEETRAQRVQLKKLPKVNKELALKLIEEEEEKQKSTWKKKVKSLPNILTDDRFKVMFENPDFQVDEESEEFRLLNPLVSKISEKRKKKLRLLEQQELREKEEEEEPEGKPSDAESSESSDDEKAWVEEVRKQRRLLQQEEKVKRQERLKEDQQTVLKPQFYEIKAGEEFRSFKDSATKQKLMNKTLEDRLKIEAKNGTLSVSDTTVGSKQLTFTLKRSEQQKKQQEAEKLHRQERKRLRRSAGHLKSRHKRGRSFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Dog
90
Pig
80
Rabbit
80
Bovine
70
Chicken
70
Xenopus
60
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BSC4 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K18 Ubiquitination
S25 Phosphorylation
K62 Ubiquitination
K74 Ubiquitination
S260 Phosphorylation
K262 Ubiquitination
K267 Ubiquitination
K296 Ubiquitination
K304 Ubiquitination
Y339 Phosphorylation
Y340 Phosphorylation
S392 Phosphorylation
K432 Sumoylation
K451 Ubiquitination
K456 Ubiquitination
K464 Ubiquitination
S475 Phosphorylation
T481 Phosphorylation
S500 Phosphorylation
S511 Phosphorylation
S514 Phosphorylation
S543 Phosphorylation
S547 Phosphorylation
S548 Phosphorylation
S550 Phosphorylation
S551 Phosphorylation
K589 Ubiquitination
Y593 Phosphorylation
K596 Ubiquitination
K605 Ubiquitination
S607 Phosphorylation
S634 Phosphorylation
S673 Phosphorylation
S686 Phosphorylation

Research Backgrounds

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the WD repeat NOL10/ENP2 family.

Restrictive clause

 

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For Research Use Only.
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