Product: ZDHHC7 Antibody
Catalog: DF4276
Description: Rabbit polyclonal antibody to ZDHHC7
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: Q9NXF8
RRID: AB_2836627

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(82%), Horse(82%), Sheep(82%)
Clonality:
Polyclonal
Specificity:
ZDHHC7 Antibody detects endogenous levels of total ZDHHC7.
RRID:
AB_2836627
Cite Format: Affinity Biosciences Cat# DF4276, RRID:AB_2836627.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHHC 7; DHHC7; FLJ10792; FLJ20279; Palmitoyltransferase ZDHHC7; ZDHHC 7; Zinc finger DHHC domain containing 7; Zinc finger DHHC domain containing protein 7; Zinc finger DHHC type containing 7; Zinc finger protein 370; ZNF 370; ZNF370;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MQPSGHRLRDVEHHPLLAENDNYDSSSSSSSEADVADRVWFIRDGCGMICAVMTWLLVAYADFVVTFVMLLPSKDFWYSVVNGVIFNCLAVLALSSHLRTMLTDPGAVPKGNATKEYMESLQLKPGEVIYKCPKCCCIKPERAHHCSICKRCIRKMDHHCPWVNNCVGEKNQRFFVLFTMYIALSSVHALILCGFQFISCVRGQWTECSDFSPPITVILLIFLCLEGLLFFTFTAVMFGTQIHSICNDETEIERLKSEKPTWERRLRWEGMKSVFGGPPSLLWMNPFVGFRFRRLPTRPRKGGPEFSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
82
Bovine
82
Sheep
82
Dog
73
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NXF8 As Substrate

Site PTM Type Enzyme
K110 Ubiquitination
K115 Ubiquitination
Y117 Phosphorylation
S120 Phosphorylation
K124 Ubiquitination
Y130 Phosphorylation
K139 Ubiquitination
K256 Ubiquitination
K259 Ubiquitination
T297 Phosphorylation
S307 Phosphorylation

Research Backgrounds

Function:

Palmitoyltransferase with broad specificity. Palmitoylates JAM3. Palmitoylates SNAP25 and DLG4/PSD95 (By similarity). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane and their function in rapid intracellular signaling upon binding of sex hormones. May play a role in follicle stimulation hormone (FSH) activation of testicular Sertoli cells (By similarity).

PTMs:

Autopalmitoylated.

Subcellular Location:

Golgi apparatus membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.