PHACTR4 Antibody - #DF4289

(3)  
Product: PHACTR4 Antibody
Catalog: DF4289
Description: Rabbit polyclonal antibody to PHACTR4
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 80 KD; 78kD(Calculated).
Uniprot: Q8IZ21
RRID: AB_2836640

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(88%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
PHACTR4 Antibody detects endogenous levels of total PHACTR4.
RRID:
AB_2836640
Cite Format: Affinity Biosciences Cat# DF4289, RRID:AB_2836640.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Phactr4; PHAR4_HUMAN; Phosphatase and actin regulator 4; PRO2963;

Immunogens

Immunogen:
Uniprot:

Q8IZ21(PHAR4_HUMAN) >>Visit HPA database.

Gene(ID):
PHACTR4(65979)
Sequence:
MEDPFEEADQPTTEPGMVLDSVEAGDTTPPTKRKSKFSGFGKIFKPWKWRKKKSSDKFKETSEVLERKISMRKPREELVKRGVLLEDPEQGGEDPGKPSDAMLKNGHTTPIGNARSSSPVQVEEEPVRLASLRKAIPEEDLKKRLGSTGSQPNSEAESVPENVPKPPLLPPKRPLSSSHEASEGQAKDATSSGGTARFIISTSITTAPAATTAATSLAKTVNLSVTPSPAPRTLPAAPASTNTTATPSLTHMVPAKQPPIPPPKPAHRNSNPVIAELSQAINSGTLLSKPSPPLPPKRGIPSTSVPTLESAAAITTKTPSDEREKSTCSMGSELLPMISPRSPSPPLPTHIPPEPPRTPPFPAKTFQVVPEIEFPPSLDLHQEIPQQEDQKKEVPKRILDQNFGEPHIPSRLPPLPLHIRIQQALTSPLPMTPILEGSHRAHSLLFENSDSFSEDSSTLGRTRSLPITIEMLKVPDDEEEEEQTCPSTFSEEMTPTSVIPKLPQCLREEEEKESDSDSEGPIQYRDEEDEDESYQSALANKVKRKDTLAMKLNHRPSEPELNLNSWPCKSKEEWNEIRHQIGNTLIRRLSQRPTPEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQRPTVAELLARKILRFNEYVEVTDAQDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVHEESKHFTRYHRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
91
Pig
88
Bovine
82
Sheep
82
Dog
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZ21 As Substrate

Site PTM Type Enzyme
T28 Phosphorylation
T31 Phosphorylation
K32 Methylation
K36 Ubiquitination
K42 Acetylation
S54 Phosphorylation
K59 Ubiquitination
S62 Phosphorylation
S70 Phosphorylation
S99 Phosphorylation
T109 Phosphorylation
S116 Phosphorylation
S117 Phosphorylation
S118 Phosphorylation
S131 Phosphorylation
S147 Phosphorylation
T148 Phosphorylation
S150 Phosphorylation
S176 Phosphorylation
S178 Phosphorylation
T195 Phosphorylation
S201 Phosphorylation
T202 Phosphorylation
S216 Phosphorylation
S224 Phosphorylation
T226 Phosphorylation
S228 Phosphorylation
S270 Phosphorylation
S288 Phosphorylation
S291 Phosphorylation
S302 Phosphorylation
S304 Phosphorylation
S332 Phosphorylation
S339 Phosphorylation
S342 Phosphorylation
S344 Phosphorylation
T349 Phosphorylation
T358 Phosphorylation
S410 Phosphorylation
S427 Phosphorylation
T432 Phosphorylation
S438 Phosphorylation
S443 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation
T462 Phosphorylation
S464 Phosphorylation
T468 Phosphorylation
T494 Phosphorylation
S514 Phosphorylation
S516 Phosphorylation
S518 Phosphorylation
Y534 Phosphorylation
S536 Phosphorylation
T547 Phosphorylation
S557 Phosphorylation
S565 Phosphorylation
K571 Ubiquitination
T584 Phosphorylation
S590 Phosphorylation
T594 Phosphorylation
T624 Phosphorylation
S628 Phosphorylation
T632 Phosphorylation
Y647 Phosphorylation
T651 Phosphorylation
Y656 Phosphorylation
T665 Phosphorylation
T668 Phosphorylation
K672 Ubiquitination
Y699 Phosphorylation

Research Backgrounds

Function:

Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway (By similarity).

Subcellular Location:

Cytoplasm. Cell projection>Lamellipodium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds PPP1CA and actin.

Family&Domains:

Belongs to the phosphatase and actin regulator family.

Restrictive clause

 

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