PITPNB Antibody - #DF4291

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Product: PITPNB Antibody
Catalog: DF4291
Description: Rabbit polyclonal antibody to PITPNB
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32 KD; 32kD(Calculated).
Uniprot: P48739
RRID: AB_2836642

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
PITPNB Antibody detects endogenous levels of total PITPNB.
RRID:
AB_2836642
Cite Format: Affinity Biosciences Cat# DF4291, RRID:AB_2836642.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000198846; OTTHUMP00000198847; Phosphatidylinositol transfer protein beta isoform; Phosphatidylinositol transfer protein, beta; PI TP beta; PI-TP-beta; PIPNB_HUMAN; Pitpnb; PtdIns transfer protein beta; PtdInsTP; PtdInsTP beta; VIB1B;

Immunogens

Immunogen:

A synthesized peptide derived from human PITPNB, corresponding to a region within C-terminal amino acids.

Uniprot:

P48739(PIPNB_HUMAN) >>Visit HPA database.

Gene(ID):
PITPNB(23760)
Expression:
P48739 PIPNB_HUMAN:

Widely expressed in various tissues including brain.

Sequence:
MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYEKDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDPALFQSVKTKRGPLGPNWKKELANSPDCPQMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNFHRQLFCWIDKWIDLTMEDIRRMEDETQKELETMRKRGSVRGTSAADV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
82
Zebrafish
60
Xenopus
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

PTMs:

Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.

Subcellular Location:

Cytoplasm. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in various tissues including brain.

Family&Domains:

Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

References

1). Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β. Signal transduction and targeted therapy, 2023 (PubMed: 37963877) [IF=40.8]

Application: WB    Species: human    Sample: HGC-27/MKN-28 cells

Fig. 5 Microcolin H functions by binding PITPα/β. a Western blot analysis of PITPα/β expression in wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2). b Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. c Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with microcolin H for 48 h. Then, the cells were cultured for 9 d, and the colonies were counted, n = 3 per group. d Western blot analysis of PITPα/β expressions for wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β). e Wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. Data are presented as the mean ± SEM. *P 

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