Product: PITPNB Antibody
Catalog: DF4291
Description: Rabbit polyclonal antibody to PITPNB
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32 KD; 32kD(Calculated).
Uniprot: P48739
RRID: AB_2836642

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
PITPNB Antibody detects endogenous levels of total PITPNB.
RRID:
AB_2836642
Cite Format: Affinity Biosciences Cat# DF4291, RRID:AB_2836642.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000198846; OTTHUMP00000198847; Phosphatidylinositol transfer protein beta isoform; Phosphatidylinositol transfer protein, beta; PI TP beta; PI-TP-beta; PIPNB_HUMAN; Pitpnb; PtdIns transfer protein beta; PtdInsTP; PtdInsTP beta; VIB1B;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P48739 PIPNB_HUMAN:

Widely expressed in various tissues including brain.

Sequence:
MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYEKDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDPALFQSVKTKRGPLGPNWKKELANSPDCPQMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNFHRQLFCWIDKWIDLTMEDIRRMEDETQKELETMRKRGSVRGTSAADV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
82
Zebrafish
60
Xenopus
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48739 As Substrate

Site PTM Type Enzyme
K5 Acetylation
K5 Ubiquitination
Y18 Phosphorylation
K31 Ubiquitination
K44 Ubiquitination
K50 Acetylation
K50 Ubiquitination
K54 Ubiquitination
Y57 Phosphorylation
K60 Ubiquitination
K65 Ubiquitination
K67 Ubiquitination
K86 Ubiquitination
Y93 Phosphorylation
T96 Phosphorylation
K104 Methylation
K104 Ubiquitination
K110 Ubiquitination
K116 Ubiquitination
R146 Methylation
Y154 Phosphorylation
K155 Ubiquitination
K167 Ubiquitination
K178 Ubiquitination
K179 Ubiquitination
S184 Phosphorylation
K201 Ubiquitination
K208 Ubiquitination
K215 Acetylation
K215 Ubiquitination
K218 Ubiquitination
R244 Methylation
T250 Phosphorylation
K252 Ubiquitination
T256 Phosphorylation
S262 Phosphorylation

Research Backgrounds

Function:

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

PTMs:

Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.

Subcellular Location:

Cytoplasm. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in various tissues including brain.

Family&Domains:

Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

Restrictive clause

 

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For Research Use Only.
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