Product: PKP2 Antibody
Catalog: DF4297
Description: Rabbit polyclonal antibody to PKP2
Application: WB IF/ICC
Cited expt.:
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 97 KD; 97kD(Calculated).
Uniprot: Q99959
RRID: AB_2836648

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PKP2 Antibody detects endogenous levels of total PKP2.
RRID:
AB_2836648
Cite Format: Affinity Biosciences Cat# DF4297, RRID:AB_2836648.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARVD 9; ARVD9; PKP 2; PKP2; PKP2_HUMAN; Plakophilin-2; Plakophilin2;

Immunogens

Immunogen:

A synthesized peptide derived from human PKP2, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q99959 PKP2_HUMAN:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

Sequence:
MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRSSVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSRQRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPVTQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADMEMTLERAVSMLEADHMLPSRISAAATFIQHECFQKSEARKRVNQLRGILKLLQLLKVQNEDVQRAVCGALRNLVFEDNDNKLEVAELNGVPRLLQVLKQTRDLETKKQITDHTVNLRSRNGWPGAVAHACNPSTLGGQGGRITRSGVRDQPDQHGLLWNLSSNDKLKNLMITEALLTLTENIIIPFSGWPEGDYPKANGLLDFDIFYNVTGCLRNMSSAGADGRKAMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQNIYIQNRNIQTDNNKSIGCFGSRSRKVKEQYQDVPMPEEKSNPKGVEWLWHSIVIRMYLSLIAKSVRNYTQEASLGALQNLTAGSGPMPTSVAQTVVQKESGLQHTRKMLHVGDPSVKKTAISLLRNLSRNLSLQNEIAKETLPDLVSIIPDTVPSTDLLIETTASACYTLNNIIQNSYQNARDLLNTGGIQKIMAISAGDAYASNKASKAASVLLYSLWAHTELHHAYKKAQFKKTDFVNSRTAKAYHSLKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May play a role in junctional plaques.

Subcellular Location:

Nucleus. Cell junction>Desmosome.
Note: Nuclear and associated with desmosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

Family&Domains:

Belongs to the beta-catenin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

References

1). Identification of PKP 2/3 as potential biomarkers of ovarian cancer based on bioinformatics and experiments. Cancer Cell International, 2020 (PubMed: 33088217) [IF=5.8]

Application: IHC    Species: Human    Sample: ovarian tissue

Fig. 2 The expression and prognostic values of PKP1/2/3 protein in ovarian cancer (Human Protein Atlas and Kaplan–Meier plotter). A The expression of PKP1 (a), PKP2 (b) and PKP3 (c) in OC and normal tissues stained by immunohistochemistry. B (a–c) Relationship between the expression of PKP1 (a), PKP2(b), PKP3(c) and OS in patients with OC; (d–f) Relationship between the expression of PKP1 (d), PKP2 (e), PKP3( f) and PFS in patients with OC; (g–i) Relationship between the expression of PKP1 (g), PKP2 (h), PKP3 (i) and OS in OC patients with TP53 mutation; (j–l) Relationship between the expression of PKP1 (j), PKP2 (k), PKP3 (l) and PFS in OC patients with TP53 mutation. PKP plakophilin, OC ovarian cancer, OS Overall Survival, PFS progression-free survival

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