PKP4 Antibody - #DF4298

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Product: PKP4 Antibody
Catalog: DF4298
Description: Rabbit polyclonal antibody to PKP4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 130 KD; 132kD(Calculated).
Uniprot: Q99569
RRID: AB_2836649

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PKP4 Antibody detects endogenous levels of total PKP4.
RRID:
AB_2836649
Cite Format: Affinity Biosciences Cat# DF4298, RRID:AB_2836649.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Catenin 4; Catenin4; FLJ31261; FLJ42243; p0071; PKP 4; PKP4; PKP4_HUMAN; Plakophilin-4; Plakophilin4;

Immunogens

Immunogen:
Uniprot:

Q99569(PKP4_HUMAN) >>Visit HPA database.

Gene(ID):
PKP4(8502)
Sequence:
MPAPEQASLVEEGQPQTRQEAASTGPGMEPETTATTILASVKEQELQFQRLTRELEVERQIVASQLERCRLGAESPSIASTSSTEKSFPWRSTDVPNTGVSKPRVSDAVQPNNYLIRTEPEQGTLYSPEQTSLHESEGSLGNSRSSTQMNSYSDSGYQEAGSFHNSQNVSKADNRQQHSFIGSTNNHVVRNSRAEGQTLVQPSVANRAMRRVSSVPSRAQSPSYVISTGVSPSRGSLRTSLGSGFGSPSVTDPRPLNPSAYSSTTLPAARAASPYSQRPASPTAIRRIGSVTSRQTSNPNGPTPQYQTTARVGSPLTLTDAQTRVASPSQGQVGSSSPKRSGMTAVPQHLGPSLQRTVHDMEQFGQQQYDIYERMVPPRPDSLTGLRSSYASQHSQLGQDLRSAVSPDLHITPIYEGRTYYSPVYRSPNHGTVELQGSQTALYRTGSVGIGNLQRTSSQRSTLTYQRNNYALNTTATYAEPYRPIQYRVQECNYNRLQHAVPADDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHQFPSVQANAAAYLQHLCFGDNKVKMEVCRLGGIKHLVDLLDHRVLEVQKNACGALRNLVFGKSTDENKIAMKNVGGIPALLRLLRKSIDAEVRELVTGVLWNLSSCDAVKMTIIRDALSTLTNTVIVPHSGWNNSSFDDDHKIKFQTSLVLRNTTGCLRNLSSAGEEARKQMRSCEGLVDSLLYVIHTCVNTSDYDSKTVENCVCTLRNLSYRLELEVPQARLLGLNELDDLLGKESPSKDSEPSCWGKKKKKKKRTPQEDQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSVGSTSSSPALLGIRDPRSEYDRTQPPMQYYNSQGDATHKGLYPGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
90
Xenopus
70
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99569 As Substrate

Site PTM Type Enzyme
S75 Phosphorylation
S77 Phosphorylation
S80 Phosphorylation
T81 Phosphorylation
S82 Phosphorylation
S83 Phosphorylation
T84 Phosphorylation
S92 Phosphorylation
K102 Ubiquitination
S106 Phosphorylation
Y114 Phosphorylation
S127 Phosphorylation
S132 Phosphorylation
S136 Phosphorylation
Y152 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
Y157 Phosphorylation
S162 Phosphorylation
S179 Phosphorylation
S203 Phosphorylation
R207 Methylation
S213 Phosphorylation
S214 Phosphorylation
S221 Phosphorylation
S223 Phosphorylation
Y224 Phosphorylation
S231 Phosphorylation
S233 Phosphorylation
R234 Methylation
S236 Phosphorylation
R254 Methylation
S259 Phosphorylation
Y261 Phosphorylation
S262 Phosphorylation
R270 Methylation
S273 Phosphorylation
Y275 Phosphorylation
S276 Phosphorylation
S281 Phosphorylation
T283 Phosphorylation
S290 Phosphorylation
S297 Phosphorylation
Y306 Phosphorylation
S314 Phosphorylation
T317 Phosphorylation
T319 Phosphorylation
T323 Phosphorylation
S327 Phosphorylation
S329 Phosphorylation
S335 Phosphorylation
S336 Phosphorylation
S337 Phosphorylation
Y369 Phosphorylation
Y372 Phosphorylation
S382 Phosphorylation
S388 Phosphorylation
S389 Phosphorylation
Y390 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S403 Phosphorylation
S406 Phosphorylation
T412 Phosphorylation
Y415 Phosphorylation
T419 Phosphorylation
Y420 Phosphorylation
Y421 Phosphorylation
S422 Phosphorylation
Y425 Phosphorylation
S427 Phosphorylation
T432 Phosphorylation
S438 Phosphorylation
T440 Phosphorylation
Y443 Phosphorylation
T445 Phosphorylation
S447 Phosphorylation
S457 Phosphorylation
S458 Phosphorylation
S461 Phosphorylation
T462 Phosphorylation
T464 Phosphorylation
Y465 Phosphorylation
Y470 Phosphorylation
T474 Phosphorylation
T475 Phosphorylation
T477 Phosphorylation
Y478 Phosphorylation
Y482 Phosphorylation
Y487 Phosphorylation
Y494 Phosphorylation
T507 Phosphorylation
S510 Phosphorylation
S512 Phosphorylation
S515 Phosphorylation
K518 Ubiquitination
K573 Ubiquitination
K588 Ubiquitination
K601 Ubiquitination
K611 Ubiquitination
K625 Acetylation
K683 Ubiquitination
K774 Ubiquitination
S776 Phosphorylation
S778 Phosphorylation
K779 Ubiquitination
T796 Phosphorylation
S812 Phosphorylation
K813 Ubiquitination
S890 Phosphorylation
S891 Phosphorylation
T894 Phosphorylation
K906 Ubiquitination
K911 Ubiquitination
K954 Ubiquitination
K970 Ubiquitination
K982 Methylation
T990 Phosphorylation
Y1001 Phosphorylation
T1013 Phosphorylation
S1016 Phosphorylation
T1017 Phosphorylation
S1048 Phosphorylation
S1049 Phosphorylation
Y1062 Phosphorylation
Y1071 Phosphorylation
Y1084 Phosphorylation
S1088 Phosphorylation
S1091 Phosphorylation
Y1094 Phosphorylation
S1097 Phosphorylation
Y1098 Phosphorylation
S1100 Phosphorylation
Y1115 Phosphorylation
Y1116 Phosphorylation
S1117 Phosphorylation
S1121 Phosphorylation
K1124 Ubiquitination
Y1129 Phosphorylation
Y1132 Phosphorylation
S1135 Phosphorylation
S1138 Phosphorylation
Y1139 Phosphorylation
Y1143 Phosphorylation
S1153 Phosphorylation
T1154 Phosphorylation
Y1156 Phosphorylation
S1157 Phosphorylation
T1158 Phosphorylation
Y1160 Phosphorylation
T1166 Phosphorylation
Y1168 Phosphorylation
Y1172 Phosphorylation
K1175 Ubiquitination
S1178 Phosphorylation
Y1179 Phosphorylation
S1187 Phosphorylation
S1190 Phosphorylation

Research Backgrounds

Function:

Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques.

Subcellular Location:

Cell junction>Desmosome. Cytoplasm>Cytoskeleton>Spindle. Midbody. Cell membrane>Peripheral membrane protein.
Note: Associated with the pericentrosomal region in interphase and with spindle poles during mitosis. In anaphase, during chromosome segregation, is recruited to the central microtubule bundle, focussed at the spindle midzone and ultimately localizes to the midbody at cytokinesis. Constituent of the midbody cytoskeletal matrix. Colocalized with desmoplakin at desmosomal junctional plaques in cultured epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PDZD2 (By similarity). Interacts (via the C-terminus) with FRMPD2 (via the PDZ 2 domain). Interacts with RHOA; the interaction is detected at the midbody. Interacts with ECT2; the interaction is detected at the midbody. Interacts with CCDC85B.

Family&Domains:

Belongs to the beta-catenin family.

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