POLDIP3 Antibody - #DF4304

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Product: POLDIP3 Antibody
Catalog: DF4304
Description: Rabbit polyclonal antibody to POLDIP3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46 KD; 46kD(Calculated).
Uniprot: Q9BY77
RRID: AB_2836655

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
POLDIP3 Antibody detects endogenous levels of total POLDIP3.
RRID:
AB_2836655
Cite Format: Affinity Biosciences Cat# DF4304, RRID:AB_2836655.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

46 kDa DNA polymerase delta interaction protein; DNA polymerase delta interacting protein 3; KIAA1649; P46; PDIP3_HUMAN; PDIP46; POLDIP3; Polymerase (DNA-directed), delta interacting protein 3; Polymerase delta interacting protein 46; Polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; SKAR;

Immunogens

Immunogen:
Uniprot:

Q9BY77(PDIP3_HUMAN) >>Visit HPA database.

Gene(ID):
POLDIP3(84271)
Sequence:
MADISLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQVPQQKAMAPLHPHPAGMRINVVNNHQAKQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALTNMSRTLVNKEEPPKELPAAEPVLSPLEGTKMTVNNLHPRVTEEDIVELFCVCGALKRARLVHPGVAEVVFVKKDDAITAYKKYNNRCLDGQPMKCNLHMNGNVITSDQPILLRLSDSPSMKKESELPRRVNSASSSNPPAEVDPDTILKALFKSSGASVTTQPTEFKIKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BY77 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
R11 Methylation
R33 Methylation
S42 Phosphorylation
S44 Phosphorylation
S63 Phosphorylation O14757 (CHEK1)
R66 Methylation
K88 Methylation
K102 Methylation
K102 Ubiquitination
T105 Phosphorylation
T106 Phosphorylation
K110 Ubiquitination
S126 Phosphorylation
S127 Phosphorylation
T140 Phosphorylation
K144 Ubiquitination
K147 Ubiquitination
K155 Ubiquitination
K178 Ubiquitination
K197 Ubiquitination
S204 Phosphorylation
S215 Phosphorylation
S217 Phosphorylation
K223 Ubiquitination
K229 Ubiquitination
Y236 Phosphorylation
T237 Phosphorylation
S244 Phosphorylation
K248 Ubiquitination
K260 Ubiquitination
K265 Ubiquitination
S275 Phosphorylation
C301 S-Nitrosylation
C303 S-Nitrosylation
K307 Acetylation
T329 Phosphorylation
K332 Ubiquitination
S368 Phosphorylation
S370 Phosphorylation
K373 Ubiquitination
S383 Phosphorylation P23443 (RPS6KB1)
S385 Phosphorylation P23443 (RPS6KB1)
S387 Phosphorylation
K400 Ubiquitination
K404 Methylation
K404 Ubiquitination
S405 Phosphorylation
S406 Phosphorylation
S409 Phosphorylation
K418 Methylation
K418 Ubiquitination

Research Backgrounds

Function:

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.

PTMs:

Phosphorylated at Ser-383 and Ser-385 by RPS6KB1.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Nucleocytoplasmic shuttling protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. Interacts with THOC2, DDX39B and ZC3H11A; the interactions are ATP-dependent and indicative for an association with the TREX complex.

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