RAB3GAP1 Antibody - #DF4371

(1)  
Product: RAB3GAP1 Antibody
Catalog: DF4371
Description: Rabbit polyclonal antibody to RAB3GAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse, Chicken
Mol.Wt.: 117 KD; 111kD(Calculated).
Uniprot: Q15042
RRID: AB_2836659

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(88%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
RAB3GAP1 Antibody detects endogenous levels of total RAB3GAP1.
RRID:
AB_2836659
Cite Format: Affinity Biosciences Cat# DF4371, RRID:AB_2836659.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434A012; KIAA0066; P130; Rab3 GAP; Rab3 GAP p130; RAB3 GTPase activating protein 130 kDa subunit; Rab3 GTPase activating protein catalytic subunit; RAB3 GTPase activating protein subunit 1 (catalytic); RAB3 GTPase-activating protein 130 kDa subunit; Rab3 GTPase-activating protein catalytic subunit; Rab3-GAP; Rab3-GAP p130; RAB3GAP; rab3gap1; RAB3GAP130; RB3GP_HUMAN; WARBM1;

Immunogens

Immunogen:
Uniprot:

Q15042(RB3GP_HUMAN) >>Visit HPA database.

Gene(ID):
RAB3GAP1(22930)
Expression:
Q15042 RB3GP_HUMAN:

Ubiquitous.

Sequence:
MAADSEPESEVFEITDFTTASEWERFISKVEEVLNDWKLIGNSLGKPLEKGIFTSGTWEEKSDEISFADFKFSVTHHYLVQESTDKEGKDELLEDVVPQSMQDLLGMNNDFPPRAHCLVRWYGLREFVVIAPAAHSDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVGECQGPGVRTDFEMVHLRKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFGACEDPISELHLATTWPHLTEGIIVDNDVYSDLDPIQAPHWSVRVRKAENPQCLLGDFVTEFFKICRRKESTDEILGRSAFEEEGKETADITHALSKLTEPASVPIHKLSVSNMVHTAKKKIRKHRGVEESPLNNDVLNTILLFLFPDAVSEKPLDGTTSTDNNNPPSESEDYNLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLASGPPDLRCCLLHQKLQMLNCCIERKKARDEGKKTSASDVTNIYPGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIHKLFVNAQRAAAMTPPEEELKRMGSPEERRQNSVSDFPPPAGREFILRTTVPRPAPYSKALPQRMYSVLTKEDFRLAGAFSSDTSFF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Chicken
88
Bovine
75
Rabbit
71
Sheep
63
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15042 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S9 Phosphorylation
K29 Ubiquitination
K38 Ubiquitination
K46 Ubiquitination
K50 Ubiquitination
T188 Phosphorylation
K197 Ubiquitination
K215 Ubiquitination
K316 Ubiquitination
K338 Ubiquitination
S340 Phosphorylation
T341 Phosphorylation
S348 Phosphorylation
K355 Ubiquitination
S365 Phosphorylation
K366 Ubiquitination
K377 Ubiquitination
S379 Phosphorylation
T386 Phosphorylation
K388 Ubiquitination
S400 Phosphorylation
K449 Ubiquitination
S450 Phosphorylation
K475 Sumoylation
K527 Acetylation
K534 Acetylation
K535 Ubiquitination
T536 Phosphorylation
S537 Phosphorylation
S539 Phosphorylation
T542 Phosphorylation
K562 Ubiquitination
S574 Phosphorylation
S579 Phosphorylation
S581 Phosphorylation
S590 Phosphorylation
S664 Phosphorylation
S676 Phosphorylation
K772 Ubiquitination
K805 Ubiquitination
K809 Ubiquitination
K817 Ubiquitination
K857 Ubiquitination
R887 Methylation
R892 Methylation
K896 Ubiquitination
T908 Phosphorylation
K915 Ubiquitination
S919 Phosphorylation
S927 Phosphorylation
S929 Phosphorylation
K953 Ubiquitination
K965 Ubiquitination

Research Backgrounds

Function:

Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.

Subcellular Location:

Cytoplasm.
Note: In neurons, it is enriched in the synaptic soluble fraction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 (By similarity). Interacts with LMAN1.

Family&Domains:

Belongs to the Rab3-GAP catalytic subunit family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.