Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [AFB1578]
Specificity:
MYST1 antibody detects endogenous levels of total MYST1.
RRID:
AB_2833603
Cite Format: Affinity Biosciences Cat# BF0328, RRID:AB_2833603.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Histone acetyltransferase KAT8; Histone acetyltransferase MYST1; hMOF; K(lysine) acetyltransferase 8; KAT 8; Lysine acetyltransferase 8; MOF; MOZ; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1; MYST 1; MYST histone acetyltransferase 1; myst protein 1; MYST-1; MYST1; MYST1_HUMAN; Ortholog of Drosophila males absent on the first (MOF); Probable histone acetyltransferase MYST1; SAS2 and TIP60 protein 1; SAS2; SAS3; TIP60 protein 1; YBF2; YBF2/SAS3; ZC2HC8;

Immunogens

Immunogen:

Purified recombinant fragment of human MYST1 expressed in E. Coli.

Uniprot:
Gene(ID):
Description:
MYST1 (MYST histone acetyltransferase 1, MOF) belongs to the MYST family of histone acetyltransferases, which are employed in the cell to bring about transcriptional regulation. The MYST family includes MYST1, is named for the founding members MOZ, yeast YBF2 and SAS2, and TIP60. All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein- protein interactions and targeting transcriptional regulators to chromatin. Although MOF is expressed in both males and females, it associates with the X chromosome only in males. MOF contains a zinc-finger domain that is used to contact the globular part of the nucleosome and histone H4. The carboxy terminal domain of human MOF also has histone acetyltransferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone
Sequence:
MAAQGAAAAVAAGTSGVAGEGEPGPGENAAAEGTAPSPGRVSPPTPARGEPEVTVEIGETYLCRRPDSTWHSAEVIQSRVNDQEGREEFYVHYVGFNRRLDEWVDKNRLALTKTVKDAVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITVDSVCLKWAPPKHKQVKLSKK

PTMs - Q9H7Z6 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T14 Phosphorylation
S15 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
Y90 Phosphorylation
K106 Ubiquitination
K113 Acetylation
K113 Ubiquitination
T114 Phosphorylation
K116 Acetylation
K125 Ubiquitination
K154 Ubiquitination
K168 Acetylation
K168 Ubiquitination
T174 Phosphorylation
K175 Ubiquitination
K177 Acetylation
K238 Acetylation
Y241 Phosphorylation
K243 Ubiquitination
K254 Ubiquitination
K257 Ubiquitination
K274 Acetylation Q9H7Z6 (KAT8)
S344 Phosphorylation
S348 Phosphorylation
K351 Acetylation
K351 Ubiquitination
T392 Phosphorylation
K410 Ubiquitination
K427 Ubiquitination
K433 Ubiquitination
K444 Ubiquitination

PTMs - Q9H7Z6 As Enzyme

Substrate Site Source
Q9H7Z6 (KAT8) K274 Uniprot

Research Backgrounds

Function:

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.

PTMs:

Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Interacts with MSL1; the interaction is direct. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain. Interacts with KANSL1; the interaction is direct. Interacts with MSL3. Interacts with NELFD (By similarity).

Family&Domains:

Belongs to the MYST (SAS/MOZ) family.

References

1). Mof regulates glucose level via altering different α-cell subset mass and intra-islet glucagon-like peptide-1, glucagon secretion. METABOLISM-CLINICAL AND EXPERIMENTAL (PubMed: 32522488) [IF=9.8]

Application: IHC    Species: human,mouse,rat    Sample: kidney

Figure 2. Mof was mainly expressed in pancreatic α -cells. IHC showed the cell location of Mof in islet was similar to glucagon, not insulin in human (A), rat (B) and mice (C) pancreas

Application: WB    Species: mouse    Sample: α-tc1-6

( E) Western blotting showed Mof protein expression s in 3, 10 and 25 mmol/L glucose in MIN6 were unchanged. ( F) Western blotting showed Mof protein expression was decreased in higher glucose concentration in α -TC1 -6

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