RFTN1 Antibody - #DF4377
| Product: | RFTN1 Antibody |
| Catalog: | DF4377 |
| Description: | Rabbit polyclonal antibody to RFTN1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Horse, Dog |
| Mol.Wt.: | 63 KD; 63kD(Calculated). |
| Uniprot: | Q14699 |
| RRID: | AB_2836665 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4377, RRID:AB_2836665.
Fold/Unfold
Cell migration-inducing gene 2 protein; KIAA0084; MIG2; PIB10; PIG9; Proliferation-inducing protein 10; Proliferation-inducing protein 9; Raft-linking protein; Raftlin; Raftlin, lipid raft linker 1; RFTN1; RFTN1_HUMAN;
Immunogens
Expressed in B-cells (at protein level) (PubMed:12805216). Expressed in dendritic cells and macrophages (PubMed:27022195, PubMed:21266579).
- Q14699 RFTN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGCGLNKLEKRDEKRPGNIYSTLKRPQVETKIDVSYEYRFLEFTTLSAAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHPFVQPTHEREKTPLEHIFRAILIKKTDRSQKTDLHNEGYILELDCCSSLDHPTDQKLIPEFIKKIQEAASQGLKFVGVIPQYHSSVNSAGSSAPVSTANSTEDARDAKNARGDHASLENEKPGTGDVCSAPAGRNQSPEPSSGPRGEVPLAKQPSSPSGEGDGGELSPQGVSKTLDGPESNPLEVHEEPLSGKMEIFTLFNKPKSHQKCRQYYPVTIPLHVSKNGQTVSGLDANWLEHMSDHFRKGGMLVNAVFYLGIVNDSLHGLTDGVFIFEAVSTEDSKTIQGYDAIVVEQWTVLEGVEVQTDYVPLLNSLAAYGWQLTCVLPTPVVKTTSEGSVSTKQIVFLQRPCLPQKIKKKESKFQWRFSREEMHNRQMRKSKGKLSARDKQQAEENEKNLEDQSSKAGDMGNCVSGQQQEGGVSEEMKGPVQEDKGEQLSPGGLLCGVGVEGEAVQNGPASHSRALVGICTGHSNPGEDARDGDAEEVRELGTVEEN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14699 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| C211 | S-Nitrosylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| S555 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in protein trafficking via association with clathrin and AP2 complex. Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production. Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (By similarity). May regulate B-cell antigen receptor-mediated signaling. May play a pivotal role in the formation and/or maintenance of lipid rafts.
Cell membrane>Lipid-anchor. Cytoplasm. Membrane raft. Endosome. Early endosome.
Note: Translocates from cytoplasm to cell membrane where it colocalizes with poly (I:C) and then moves to endosomes where it colocalizes with TLR3 (PubMed:21266579). Translocates from cytoplasm to cell membrane where it colocalizes with TLR4 and then together with TLR4 moves to endosomes, upon lipopolysaccharide stimulation (PubMed:27022195).
Expressed in B-cells (at protein level). Expressed in dendritic cells and macrophages.
Interacts with TLR4; the interaction occurs in response to lipopolysaccharide stimulation. Interacts with CLTC; the interaction occurs in response to pathogens. Interacts with AP2A1 and AP2B1.
Belongs to the raftlin family.
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