DDX52 Antibody | Affinity Biosciences

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DDX52 Antibody - Western blot analysis of extracts from HepG2 cells using DDX52 antibody.

Product:	DDX52 Antibody
Catalog:	DF4333
Description:	Rabbit polyclonal antibody to DDX52
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	67 KD; 67kD(Calculated).
Uniprot:	Q9Y2R4
RRID:	AB_2836701

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(89%), Horse(89%), Sheep(89%)

Clonality:

Polyclonal

Specificity:

DDX52 Antibody detects endogenous levels of total DDX52.

RRID:

AB_2836701
Cite Format: Affinity Biosciences Cat# DF4333, RRID:AB_2836701.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP dependent RNA helicase ROK1 like; ATP-dependent RNA helicase ROK1-like; DDX52; DDX52_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 52; DEAD box protein 52; Human sequence similar to yeast 19; HUSSY19; Probable ATP dependent RNA helicase DDX52; Probable ATP-dependent RNA helicase DDX52; ROK1; ROK1, S. cerevisiae, homolog of;

Immunogens

Immunogen:

Uniprot:

Q9Y2R4(DDX52_HUMAN) >>Visit HPA database.

Gene(ID):

DDX52(11056)

Sequence:

Q9Y2R4 DDX52_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVHDLFRRLGAGAKFDTRRFSADAARFQIGKRKYDFDSSEVLQGLDFFGNKKSVPGVCGASQTHQKPQNGEKKEESLTERKREQSKKKRKTMTSEIASQEEGATIQWMSSVEAKIEDKKVQRESKLTSGKLENLRKEKINFLRNKHKIHVQGTDLPDPIATFQQLDQEYKINSRLLQNILDAGFQMPTPIQMQAIPVMLHGRELLASAPTGSGKTLAFSIPILMQLKQPANKGFRALIISPTRELASQIHRELIKISEGTGFRIHMIHKAAVAAKKFGPKSSKKFDILVTTPNRLIYLLKQDPPGIDLASVEWLVVDESDKLFEDGKTGFRDQLASIFLACTSHKVRRAMFSATFAYDVEQWCKLNLDNVISVSIGARNSAVETVEQELLFVGSETGKLLAMRELVKKGFNPPVLVFVQSIERAKELFHELIYEGINVDVIHAERTQQQRDNTVHSFRAGKIWVLICTALLARGIDFKGVNLVINYDFPTSSVEYIHRIGRTGRAGNKGKAITFFTEDDKPLLRSVANVIQQAGCPVPEYIKGFQKLLSKQKKKMIKKPLERESISTTPKCFLEKAKDKQKKVTGQNSKKKVALEDKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Pig

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2R4 As Substrate

Q9Y2R4

Site	PTM Type	Enzyme	Source
K15	Acetylation		Uniprot
S22	Phosphorylation	Q96GD4 (AURKB)	Uniprot
R27	Methylation		Uniprot
Y35	Phosphorylation		Uniprot
S39	Phosphorylation		Uniprot
S40	Phosphorylation		Uniprot
K52	Ubiquitination		Uniprot
K53	Ubiquitination		Uniprot
S99	Phosphorylation		Uniprot
T105	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
S111	Phosphorylation		Uniprot
K131	Acetylation		Uniprot
K131	Ubiquitination		Uniprot
K139	Ubiquitination		Uniprot
K148	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K228	Ubiquitination		Uniprot
K270	Ubiquitination		Uniprot
T291	Phosphorylation		Uniprot
T292	Phosphorylation		Uniprot
K328	Ubiquitination		Uniprot
K409	Ubiquitination		Uniprot
K511	Ubiquitination		Uniprot
K521	Ubiquitination		Uniprot
K543	Ubiquitination		Uniprot
S565	Phosphorylation		Uniprot
T569	Phosphorylation		Uniprot
K571	Ubiquitination		Uniprot

Research Backgrounds

Q9Y2R4_DDX52_HUMAN

Subcellular Location:

Nucleus>Nucleolus.

Family&Domains:

Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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DDX52 Antibody - #DF4333