FAM35A/B Antibody | Affinity Biosciences

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FAM35A/B Antibody - Western blot analysis of extracts from Jurkat cells, using FAM35A/B antibody.

Product:	FAM35A/B Antibody
Catalog:	DF4338
Description:	Rabbit polyclonal antibody to FAM35A/B
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	94 KD; 94kD(Calculated).
Uniprot:	Q86V20
RRID:	AB_2836706

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

FAM35A/B Antibody detects endogenous levels of total FAM35A/B.

RRID:

AB_2836706
Cite Format: Affinity Biosciences Cat# DF4338, RRID:AB_2836706.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bA163M19.1; FA35A_HUMAN; FAM35A; FAM35A1; Family with sequence similarity 35 member A; Hypothetical protein LOC54537; MGC5560; Protein FAM35A;

Immunogens

Immunogen:

Uniprot:

Q86V20(SHLD2_HUMAN) >>Visit HPA database.

Gene(ID):

FAM35A(54537)

Sequence:

Q86V20 SHLD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGGSQVHIFWGAPIAPLKITVSEDTASLMSVADPWKKIQLLYSQHSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFVRSVSETQNIESQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQPKHQPDICGKNFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQCLGLFSSNAVDKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNKGNVNMETEPKASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRIHINSDKGLEEHTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSEDKVGQSEALSRVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQKYNCLVMVLSPCHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLWRTAAFWAFTVFLGDIILLTDVVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDLLAYVSSKHSYLRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSYRGQKQKKVMLTVEQAQDQHYALVLWGPGAAWYPQLQRKKGVVLIKAQISELAFPITASQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSCLPFTMKKIYYRPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYGMVVADLFHSLLAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL

PTMs - Q86V20 As Substrate

Q86V20

Site	PTM Type	Source
K63	Ubiquitination	Uniprot
S70	Phosphorylation	Uniprot
K89	Ubiquitination	Uniprot
K106	Ubiquitination	Uniprot
S109	Phosphorylation	Uniprot
K137	Ubiquitination	Uniprot
K140	Ubiquitination	Uniprot
K221	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
S339	Phosphorylation	Uniprot
K385	Ubiquitination	Uniprot
K400	Ubiquitination	Uniprot
S465	Phosphorylation	Uniprot
T473	Phosphorylation	Uniprot
S480	Phosphorylation	Uniprot
S566	Phosphorylation	Uniprot
Y567	Phosphorylation	Uniprot
K622	Acetylation	Uniprot
K653	Acetylation	Uniprot
S683	Phosphorylation	Uniprot
S787	Phosphorylation	Uniprot
S792	Phosphorylation	Uniprot

Research Backgrounds

Q86V20_SHLD2_HUMAN

Function:

Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection. Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres.

Subcellular Location:

Nucleus. Chromosome.
Note: Localizes to nuclear foci in response to DNA damage.

Subunit Structure:

Component of the shieldin complex, consisting of SHLD1, SHLD2, SHLD3 and MAD2L2/REV7. Within the complex, SHLD2 forms a scaffold which interacts with a SHLD3-MAD2L2 subcomplex via its N-terminus, and with SHLD1 via its C-terminus. Interacts with TP53BP1. Interacts with RIF1.

Family&Domains:

Belongs to the SHLD2 family.

Restrictive clause

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FAM35A/B Antibody - #DF4338