PPP1R16B Antibody - #DF4342
| Product: | PPP1R16B Antibody |
| Catalog: | DF4342 |
| Description: | Rabbit polyclonal antibody to PPP1R16B |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 64 KD; 64kD(Calculated). |
| Uniprot: | Q96T49 |
| RRID: | AB_2836710 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4342, RRID:AB_2836710.
Fold/Unfold
ANKRD4; Ankyrin repeat domain containing protein 4; ankyrin repeat domain protein 4; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; hTIMAP; KIAA0823; PP16B_HUMAN; PPP1R16B; Protein phosphatase 1 regulatory inhibitor subunit 16B; protein phosphatase 1, regulatory (inhibitor) subunit 16B; TGF beta inhibited membrane associated protein; TGF-beta-inhibited membrane-associated protein; TIMAP;
Immunogens
- Q96T49 PP16B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96T49 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y382 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| Y437 | Phosphorylation | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| Y536 | Phosphorylation | Uniprot | |
| Y537 | Phosphorylation | Uniprot | |
| K550 | Ubiquitination | Uniprot |
Research Backgrounds
Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation. Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the dephosphorylation of EEF1A1.
Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity (By similarity).
Cell membrane. Cell membrane>Lipid-anchor. Nucleus. Cell projection.
Note: Colocalizes with RPSA/LAMR1 in the cell membrane (PubMed:16263087). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (PubMed:25007873).
Interacts with PPP1CA, PPP1CB and MSN. Interacts (via its fourth ankyrin repeat) with the mature dimeric form of RPSA/LAMR1. Interacts with EEF1A1. Interacts with PTEN. Interacts with ECE1 (By similarity).
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