RED Antibody - #DF4352

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Product: RED Antibody
Catalog: DF4352
Description: Rabbit polyclonal antibody to RED
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 66 KD; 66kD(Calculated).
Uniprot: Q13123
RRID: AB_2836720

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(87%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(93%), Xenopus(93%)
Clonality:
Polyclonal
Specificity:
RED Antibody detects endogenous levels of total RED.
RRID:
AB_2836720
Cite Format: Affinity Biosciences Cat# DF4352, RRID:AB_2836720.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

chondrosarcoma associated protein 2; CSA2; Cytokine IK; IK; IK cytokine down regulator of HLA II; IK factor; IK Gene; prer protein; Protein Red; Protein RER; RD element; RED protein; RED_HUMAN; RER;

Immunogens

Immunogen:
Uniprot:

Q13123(RED_HUMAN) >>Visit HPA database.

Gene(ID):
IK(3550)
Expression:
Q13123 RED_HUMAN:

Ubiquitous.

Sequence:
MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNVYRMLFKSKAYERNELFLPGRMAYVVDLDDEYADTDIPTTLIRSKADCPTMEAQTTLTTNDIVISKLTQILSYLRQGTRNKKLKKKDKGKLEEKKPPEADMNIFEDIGDYVPSTTKTPRDKERERYRERERDRERDRDRDRERERERDRERERERDREREEEKKRHSYFEKPKVDDEPMDVDKGPGSTKELIKSINEKFAGSAGWEGTESLKKPEDKKQLGDFFGMSNSYAECYPATMDDMAVDSDEEVDYSKMDQGNKKGPLGRWDFDTQEEYSEYMNNKEALPKAAFQYGIKMSEGRKTRRFKETNDKAELDRQWKKISAIIEKRKKMEADGVEVKRPKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
93
Chicken
93
Zebrafish
87
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13123 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
S10 Phosphorylation
K30 Ubiquitination
K38 Sumoylation
K38 Ubiquitination
T42 Phosphorylation
T48 Phosphorylation
K54 Acetylation
K54 Ubiquitination
K83 Acetylation
K83 Ubiquitination
K98 Acetylation
K98 Ubiquitination
K112 Ubiquitination
Y114 Phosphorylation
T117 Phosphorylation
K137 Acetylation
S138 Phosphorylation
K142 Ubiquitination
K151 Sumoylation
K151 Ubiquitination
K164 Ubiquitination
K173 Sumoylation
K173 Ubiquitination
K191 Ubiquitination
K198 Ubiquitination
K205 Sumoylation
K205 Ubiquitination
K209 Ubiquitination
K224 Sumoylation
Y247 Phosphorylation
K260 Ubiquitination
T270 Phosphorylation
S287 Phosphorylation
K303 Acetylation
K305 Acetylation
K310 Sumoylation
K310 Ubiquitination
Y325 Phosphorylation
S328 Phosphorylation
T329 Phosphorylation
T330 Phosphorylation
K331 Ubiquitination
T332 Phosphorylation
S382 Phosphorylation
Y383 Phosphorylation
K386 Acetylation
K388 Sumoylation
K404 Sumoylation
K404 Ubiquitination
K408 Acetylation
K408 Sumoylation
K408 Ubiquitination
S409 Phosphorylation
K413 Sumoylation
K413 Ubiquitination
S417 Phosphorylation
T452 Phosphorylation
S460 Phosphorylation
Y466 Phosphorylation
T485 Phosphorylation
Y489 Phosphorylation
Y492 Phosphorylation
K501 Sumoylation
K501 Ubiquitination
Y506 Phosphorylation
K509 Ubiquitination
K520 Methylation
K520 Ubiquitination
R530 Methylation
K534 Ubiquitination
S536 Phosphorylation
K541 Ubiquitination
K544 Sumoylation
K553 Sumoylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing as a component of the spliceosome. Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species (Probable). Required for normal mitotic cell cycle progression. Recruits MAD1L1 and MAD2L1 to kinetochores, and is required to trigger the spindle assembly checkpoint. Required for normal accumulation of SMU1.

(Microbial infection) Required, together with SMU1, for normal splicing of influenza A virus NS1 pre-mRNA, which is required for the production of the exportin NS2 and for the production of influenza A virus particles. Not required for the production of VSV virus particles.

Subcellular Location:

Nucleus. Nucleus>Nucleoplasm. Chromosome. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Predominantly present throughout the nucleoplasm during prometaphase, metaphase and anaphase. Is also detected in nuclear foci that are not identical with Cajal bodies. Starts to accumulate at chromosomes during telophase, and is nearly exclusively associated with chromosomes in newly divided cells (PubMed:24252166). Colocalizes with MAD1L1 at mitotic spindle poles during metaphase and anaphase (PubMed:22351768).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

(Microbial infection) Identified in a complex with SMU1 and influenza A virus RNA polymerase subunits PB1 and PB2. Directly interacts with SMU1 and with influenza A virus RNA polymerase subunits PB1 and PB2.

Component of the spliceosome B complex. Interacts with SMU1. Interacts with MAD1L1. May interact with DHX15.

Family&Domains:

Belongs to the RED family.

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