PLEKHG4 Antibody - #DF4358

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Product: PLEKHG4 Antibody
Catalog: DF4358
Description: Rabbit polyclonal antibody to PLEKHG4
Application: WB IF/ICC
Reactivity: Human
Prediction: Mouse, Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 135 KD; 131kD(Calculated).
Uniprot: Q58EX7
RRID: AB_2836726

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Mouse(%), Pig(90%), Bovine(90%), Horse(90%), Sheep(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
PLEKHG4 Antibody detects endogenous levels of total PLEKHG4.
RRID:
AB_2836726
Cite Format: Affinity Biosciences Cat# DF4358, RRID:AB_2836726.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGEF44; DKFZp434I216; PH domain containing family G member 4; PH domain-containing family G member 4; PKHG4_HUMAN; Pleckstrin homology and RhoGEF domain containing G4; Pleckstrin homology domain containing family G (with RhoGef domain) member 4; Pleckstrin homology domain containing family G member 4; Pleckstrin homology domain-containing family G member 4; PLEKHG 4; PLEKHG4; PRTPHN 1; PRTPHN1; Puratrophin 1; Puratrophin-1; Puratrophin1; Purkinje cell atrophy associated protein 1; Purkinje cell atrophy-associated protein 1; SCA 4; SCA4;

Immunogens

Immunogen:
Uniprot:

Q58EX7(PKHG4_HUMAN) >>Visit HPA database.

Gene(ID):
PLEKHG4(25894)
Expression:
Q58EX7 PKHG4_HUMAN:

Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.

Sequence:
MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVKDPGPPRPPAGATQDEELQGSPLSRKFQLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSGLSPGALDSDPVGLGDPLSEISKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLLLCAHSPAWLQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSPDYRTAMDKADELYDRVDGLLHQLTLQSNQRIQALELVQTLEARESGLHQIEVWLQQVGWPALEEAGEPSLDMLLQAQGSFQELYQVAQEQVRQGEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQLFREALTWAEEGQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQECRWAWARCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNLGQSLSEPACHCHHAATIAACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEMVATEREYVRALEYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHCHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALGDHLDLASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKRSFKMADLGLTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDHDSLYLGASNSLPGDPASCSVLGSLNLHLYRDPALLGLRCPLYPSFPEEAALEAEAELGGQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPCV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
80
Dog
80
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q58EX7 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S14 Phosphorylation
S64 Phosphorylation
S67 Phosphorylation
K69 Ubiquitination
S142 Phosphorylation
K405 Ubiquitination
T419 Phosphorylation
K423 Ubiquitination
Y428 Phosphorylation
K511 Ubiquitination
K608 Ubiquitination
K642 Ubiquitination
K648 Ubiquitination
K674 Ubiquitination
S677 Phosphorylation
S687 Phosphorylation
S716 Phosphorylation
K784 Ubiquitination
K844 Ubiquitination
K846 Ubiquitination
K862 Ubiquitination
K869 Ubiquitination
K922 Ubiquitination
Y970 Phosphorylation
K971 Ubiquitination
K975 Ubiquitination
K1049 Ubiquitination
S1057 Phosphorylation
R1064 Methylation
Y1068 Phosphorylation
K1071 Ubiquitination
C1190 S-Nitrosylation

Research Backgrounds

Function:

Possible role in intracellular signaling and cytoskeleton dynamics at the Golgi.

Tissue Specificity:

Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.

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