RAB20 Antibody - #DF4397

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Product: RAB20 Antibody
Catalog: DF4397
Description: Rabbit polyclonal antibody to RAB20
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 28 KD; 26kD(Calculated).
Uniprot: Q9NX57
RRID: AB_2836752

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RAB20 Antibody detects endogenous levels of total RAB20.
RRID:
AB_2836752
Cite Format: Affinity Biosciences Cat# DF4397, RRID:AB_2836752.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ20429; RAB20; RAB20 member RAS oncogene family; RAB20_HUMAN; Ras related protein Rab20; Ras-related protein Rab-20;

Immunogens

Immunogen:
Uniprot:

Q9NX57(RAB20_HUMAN) >>Visit HPA database.

Gene(ID):
RAB20(55647)
Expression:
Q9NX57 RAB20_HUMAN:

Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).

Sequence:
MRKPDSKIVLLGDMNVGKTSLLQRYMERRFPDTVSTVGGAFYLKQWRSYNISIWDTAGREQFHGLGSMYCRGAAAIILTYDVNHRQSLVELEDRFLGLTDTASKDCLFAIVGNKVDLTEEGALAGQEKEECSPNMDAGDRVSPRAPKQVQLEDAVALYKKILKYKMLDEQDVPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMILQQRAERPSHTVDISSHKPPKRTRSGCCA

PTMs - Q9NX57 As Substrate

Site PTM Type Enzyme
Y25 Phosphorylation
S132 Phosphorylation
S142 Phosphorylation
K147 Ubiquitination
K159 Ubiquitination

Research Backgrounds

Function:

Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.

Subcellular Location:

Golgi apparatus. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side.
Note: Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Restrictive clause

 

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