Product: RAB2B Antibody
Catalog: DF4400
Description: Rabbit polyclonal antibody to RAB2B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 24 KD; 24kD(Calculated).
Uniprot: Q8WUD1
RRID: AB_2836755

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RAB2B Antibody detects endogenous levels of total RAB2B.
RRID:
AB_2836755
Cite Format: Affinity Biosciences Cat# DF4400, RRID:AB_2836755.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ14824; GTP binding protein RAB2B; GTP-binding protein RAB2B; RAB2B; RAB2B member RAS oncogene family; RAB2B_HUMAN; RAS family, member RAB2B; ras related protein Rab 2B; ras related protein Rab2B; Ras-related protein Rab-2B;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8WUD1 RAB2B_HUMAN:

Expressed in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta. Not detected in heart, brain, spleen, testis, ovary, small intestine and leukocyte.

Sequence:
MTYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMVNIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLTSWLEDARQHSSSNMVIMLIGNKSDLESRRDVKREEGEAFAREHGLIFMETSAKTACNVEEAFINTAKEIYRKIQQGLFDVHNEANGIKIGPQQSISTSVGPSASQRNSRDIGSNSGCC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WUD1 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
Y5 Phosphorylation
Y9 Phosphorylation
T15 Phosphorylation
K56 Ubiquitination
S67 Phosphorylation
S70 Phosphorylation
T72 Phosphorylation
Y86 Phosphorylation
T89 Phosphorylation
K165 Ubiquitination
S192 Phosphorylation
S196 Phosphorylation
S202 Phosphorylation

Research Backgrounds

Function:

Required for protein transport from the endoplasmic reticulum to the Golgi complex.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Endoplasmic reticulum membrane. Golgi apparatus membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta. Not detected in heart, brain, spleen, testis, ovary, small intestine and leukocyte.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.