RGS12 Antibody | Affinity Biosciences

1/1

RGS12 Antibody - Western blot analysis of extracts from Rat brain, using RGS12 Antibody at 1/1000 dilution.

Product:	RGS12 Antibody
Catalog:	DF4415
Description:	Rabbit polyclonal antibody to RGS12
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Sheep, Dog
Mol.Wt.:	156 KD; 156kD(Calculated).
Uniprot:	O14924
RRID:	AB_2836770

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

RGS12 Antibody detects endogenous levels of total RGS12.

RRID:

AB_2836770
Cite Format: Affinity Biosciences Cat# DF4415, RRID:AB_2836770.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Regulator of G protein signalling 12; RGS12; RGS12_HUMAN; RGS 12;

Immunogens

Immunogen:

Uniprot:

O14924(RGS12_HUMAN) >>Visit HPA database.

Gene(ID):

RGS12(6002)

Expression:

O14924 RGS12_HUMAN:

Isoform 3 is brain specific.

Sequence:

O14924 RGS12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWLKPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFDVGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIELPSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFGFECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSSNSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDPEGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPVHVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKREWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITRSLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSSILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSGEKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEERTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQWEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQSQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGSGTHGSRDLPVNRIIDVDLVTGSAPGRDGGIAGAQAGPGRSQASGGPPTSDLPGLGPVPGEPAKPKTSAHHATFV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Horse

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14924 As Substrate

O14924

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
K82	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
S107	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot
S172	Phosphorylation	Uniprot
S195	Phosphorylation	Uniprot
K196	Ubiquitination	Uniprot
K287	Ubiquitination	Uniprot
S435	Phosphorylation	Uniprot
S483	Phosphorylation	Uniprot
T492	Phosphorylation	Uniprot
R524	Methylation	Uniprot
R633	Methylation	Uniprot
R643	Methylation	Uniprot
S649	Phosphorylation	Uniprot
S661	Phosphorylation	Uniprot
S671	Phosphorylation	Uniprot
T676	Phosphorylation	Uniprot
S694	Phosphorylation	Uniprot
S697	Phosphorylation	Uniprot
S700	Phosphorylation	Uniprot
S711	Phosphorylation	Uniprot
S715	Phosphorylation	Uniprot
K777	Ubiquitination	Uniprot
K824	Ubiquitination	Uniprot
S850	Phosphorylation	Uniprot
S853	Phosphorylation	Uniprot
S856	Phosphorylation	Uniprot
T866	Phosphorylation	Uniprot
S874	Phosphorylation	Uniprot
S876	Phosphorylation	Uniprot
S879	Phosphorylation	Uniprot
S889	Phosphorylation	Uniprot
S909	Phosphorylation	Uniprot
S934	Phosphorylation	Uniprot
S940	Phosphorylation	Uniprot
S947	Phosphorylation	Uniprot
K962	Acetylation	Uniprot
K979	Acetylation	Uniprot
K1082	Ubiquitination	Uniprot
K1115	Ubiquitination	Uniprot
K1122	Ubiquitination	Uniprot
S1148	Phosphorylation	Uniprot
K1211	Ubiquitination	Uniprot
S1294	Phosphorylation	Uniprot
S1304	Phosphorylation	Uniprot
S1321	Phosphorylation	Uniprot
T1331	Phosphorylation	Uniprot

Research Backgrounds

O14924_RGS12_HUMAN

Function:

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.

Behaves as a cell cycle-dependent transcriptional repressor, promoting inhibition of S-phase DNA synthesis.

Subcellular Location:

Nucleus. Cytoplasm. Cell projection>Dendrite. Cell junction>Synapse.

Nucleus matrix.
Note: Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains.

Tissue Specificity:

Isoform 3 is brain specific.

Subunit Structure:

Interacts with GNAI1. Interacts with GNAI2 and GNAI3; the interactions are GDP-dependent (By similarity).

Family&Domains:

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

References

1). RGS12 is a target of penehyclidine hydrochloride that enhances oxidative stress and ferroptosis in a model of myocardial ischemia/reperfusion injury by inhibiting the Nrf2 pathway. International journal of molecular medicine, 2025 (PubMed: 39930821) [IF=5.7]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

RGS12 Antibody - #DF4415