RGS12 Antibody | Affinity Biosciences

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RGS12 Antibody - Western blot analysis of extracts from Rat brain, using RGS12 Antibody at 1/1000 dilution.

$Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells. (A) The MIRI model was developed using a 30 min ischemia and 24 h reperfusion. (B) Echocardiogram, LVEF and LVFS of mice. (C) Quantification of the infarct area in the myocardial tissue and images of infarct tissue. (D) Serum LDH and CK activity. (E) ROS levels (magnification, ×400) and (F) Fe2+ content in the myocardial tissue. RGS12 expression levels in the myocardial tissue was analyzed using (G) reverse-transcription quantitative PCR and western blotting and (H) immunofluorescence. Magnification, ×400. n=6. (I) A cell model of H/R-induced injury was developed using a 12 h serum starvation, 8 h hypoxia and 12 h reoxygenation. RGS12 expression levels in H/R cells was analyzed using (J) reverse transcription quantitative PCR and western blotting and (K) immunofluorescence. Magnification, ×400. n=3. Data were presented as the mean ± SD. MIRI, myocardial ischemia/reperfusion injury; H/R, hypoxia/reoxygenation; RGS12, regulator of G-protein signaling 12; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LDH, lactate dehydrogenase; CK, creatine kinase; ROS, reactive oxygen species.$

RGS12 Antibody - Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells.

Product:	RGS12 Antibody
Catalog:	DF4415
Description:	Rabbit polyclonal antibody to RGS12
Application:	WB IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Sheep, Dog
Mol.Wt.:	156 KD; 156kD(Calculated).
Uniprot:	O14924
RRID:	AB_2836770

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

RGS12 Antibody detects endogenous levels of total RGS12.

RRID:

AB_2836770
Cite Format: Affinity Biosciences Cat# DF4415, RRID:AB_2836770.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Regulator of G protein signalling 12; RGS12; RGS12_HUMAN; RGS 12;

Immunogens

Immunogen:

A synthesized peptide derived from human RGS12, corresponding to a region within N-terminal amino acids.

Uniprot:

O14924(RGS12_HUMAN) >>Visit HPA database.

Gene(ID):

RGS12(6002)

Expression:

O14924 RGS12_HUMAN:

Isoform 3 is brain specific.

Sequence:

O14924 RGS12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWLKPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFDVGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIELPSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFGFECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSSNSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDPEGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPVHVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKREWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITRSLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSSILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSGEKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEERTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQWEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQSQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGSGTHGSRDLPVNRIIDVDLVTGSAPGRDGGIAGAQAGPGRSQASGGPPTSDLPGLGPVPGEPAKPKTSAHHATFV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Horse

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O14924_RGS12_HUMAN

Function:

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.

Behaves as a cell cycle-dependent transcriptional repressor, promoting inhibition of S-phase DNA synthesis.

Subcellular Location:

Nucleus. Cytoplasm. Cell projection>Dendrite. Cell junction>Synapse.

Nucleus matrix.
Note: Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains.

Tissue Specificity:

Isoform 3 is brain specific.

Family&Domains:

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

References

1). RGS12 is a target of penehyclidine hydrochloride that enhances oxidative stress and ferroptosis in a model of myocardial ischemia/reperfusion injury by inhibiting the Nrf2 pathway. International journal of molecular medicine, 2025 (PubMed: 39930821) [IF=5.7]

Application: WB Species: Mouse Sample:

Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells. (A) The MIRI model was developed using a 30 min ischemia and 24 h reperfusion. (B) Echocardiogram, LVEF and LVFS of mice. (C) Quantification of the infarct area in the myocardial tissue and images of infarct tissue. (D) Serum LDH and CK activity. (E) ROS levels (magnification, ×400) and (F) Fe2+ content in the myocardial tissue. RGS12 expression levels in the myocardial tissue was analyzed using (G) reverse-transcription quantitative PCR and western blotting and (H) immunofluorescence. Magnification, ×400. n=6. (I) A cell model of H/R-induced injury was developed using a 12 h serum starvation, 8 h hypoxia and 12 h reoxygenation. RGS12 expression levels in H/R cells was analyzed using (J) reverse transcription quantitative PCR and western blotting and (K) immunofluorescence. Magnification, ×400. n=3. Data were presented as the mean ± SD. MIRI, myocardial ischemia/reperfusion injury; H/R, hypoxia/reoxygenation; RGS12, regulator of G-protein signaling 12; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LDH, lactate dehydrogenase; CK, creatine kinase; ROS, reactive oxygen species.

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RGS12 Antibody - #DF4415