Product: RGS5 Antibody
Catalog: DF4417
Description: Rabbit polyclonal antibody to RGS5
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 21 KD; 21kD(Calculated).
Uniprot: O15539
RRID: AB_2836772

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
RGS5 Antibody detects endogenous levels of total RGS5.
RRID:
AB_2836772
Cite Format: Affinity Biosciences Cat# DF4417, RRID:AB_2836772.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129; Regulator of G Protein Signalling 5; Regulator of G-protein signaling 5; RGS 5; RGS5; RGS5_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human RGS5, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Sequence:
MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
86
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha (By similarity).

Subcellular Location:

Cytoplasm. Membrane.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

References

1). RGS5+ lymphatic endothelial cells facilitate metastasis and acquired drug resistance of breast cancer through oxidative stress-sensing mechanism. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 2024 (PubMed: 39306871) [IF=15.8]

2). ERG mediates the differentiation of hepatic progenitor cells towards immunosuppressive PDGFRα+ cancer-associated fibroblasts during hepatocarcinogenesis. Cell death & disease, 2025 (PubMed: 39827226) [IF=8.1]

Application: IF/ICC    Species: Rat    Sample: HPCs

Fig. 4: Lipopolysaccharide induces the differentiation of HPCs towards PDGFRα+ CAFs. A, B Pseudotime trajectories of all CAFs revealing 3 branches. The blue, red, green and grey circles indicate cells at the termini of the branches. C Heatmap showing dynamic changes in gene expression in the CAF lineage. The genes were clustered into 3 gene sets, each of which was characterized by specific expression profiles, as depicted by a selection of genes characteristic of each cluster. Rows: GO enrichment analysis of DEGs; columns: cells shown in pseudotime order. D Expression of key regulators plotted along the pseudotime axis. E, F The expression of CAF markers (α-SMA, RGS5, and PDGFRα) was detected in HPCs treated with LPS for 7 days and 14 days by immunofluorescence staining. Nuclei were stained with DAPI (blue). G Fuzzy Cmeans clustering identified 8 distinct temporal patterns of gene expression. The x-axis shows three stages of HPCs (control and treated with LPS for 7 days and 14 days), whereas the y-axis shows the log2-transformed, normalized intensity ratios at each stage. (H Bar graphs showing the pathways identified after KEGG and GO enrichment analysis of genes in Cluster 4 and 5, respectively. I, J Volcano plot showing differentially expressed genes (DEGs) between HPCs treated with LPS for 7 days and untreated HPCs (I, left panel) or between HPCs treated with LPS for 14 days and untreated HPCs. (J left panel). The bar graphs show the Gene Ontology (GO) functional enrichment analysis results for the DEGs between the group treated with LPS for 7 days and the control group and between the group treated with LPS for 14 days and the control group.

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For Research Use Only.
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