RHG18 Antibody - #DF4427

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Product: RHG18 Antibody
Catalog: DF4427
Description: Rabbit polyclonal antibody to RHG18
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Mouse, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 75 KD; 75kD(Calculated).
Uniprot: Q8N392
RRID: AB_2836782

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Mouse(%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
RHG18 Antibody detects endogenous levels of total RHG18.
RRID:
AB_2836782
Cite Format: Affinity Biosciences Cat# DF4427, RRID:AB_2836782.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4833419J07Rik; AI314906; AI843501; Arhgap18; bA307O14.2; FLJ25728; MacGAP; MGC126757; MGC138145; OTTHUMP00000017190; OTTMUSP00000028606; RHG18_HUMAN; Rho GTPase-activating protein 18; Rho-type GTPase-activating protein 18; SENEX;

Immunogens

Immunogen:
Uniprot:

Q8N392(RHG18_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP18(93663)
Sequence:
MSWLSSSQGVVLTAYHPSGKDQTVGNSHAKAGEEATSSRRYGQYTMNQESTTIKVMEKPPFDRSISQDSLDELSMEDYWIELENIKKSSENSQEDQEVVVVKEPDEGELEEEWLKEAGLSNLFGESAGDPQESIVFLSTLTRTQAAAVQKRVETVSQTLRKKNKQYQIPDVRDIFAQQRESKETAPGGTESQSLRTNENKYQGRDDEASNLVGEEKLIPPEETPAPETDINLEVSFAEQALNQKESSKEKIQKSKGDDATLPSFRLPKDKTGTTRIGDLAPQDMKKVCHLALIELTALYDVLGIELKQQKAVKIKTKDSGLFCVPLTALLEQDQRKVPGMRIPLIFQKLISRIEERGLETEGLLRIPGAAIRIKNLCQELEAKFYEGTFNWESVKQHDAASLLKLFIRELPQPLLSVEYLKAFQAVQNLPTKKQQLQALNLLVILLPDANRDTLKALLEFLQRVIDNKEKNKMTVMNVAMVMAPNLFMCHALGLKSSEQREFVMAAGTANTMHLLIKYQKLLWTIPKFIVNQVRKQNTENHKKDKRAMKKLLKKMAYDREKYEKQDKSTNDADVPQGVIRVQAPHLSKVSMAIQLTEELKASDVLARFLSQESGVAQTLKKGEVFLYEIGGNIGERCLDDDTYMKDLYQLNPNAEWVIKSKPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Sheep
88
Chicken
83
Zebrafish
63
Xenopus
57
Pig
0
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N392 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T36 Phosphorylation
Y44 Phosphorylation
S64 Phosphorylation
S66 Phosphorylation
S69 Phosphorylation
S92 Phosphorylation
T154 Phosphorylation
T158 Phosphorylation
S209 Phosphorylation
K255 Ubiquitination
T260 Phosphorylation
S263 Phosphorylation
K310 Acetylation
K395 Ubiquitination
T431 Phosphorylation
K432 Acetylation
K432 Ubiquitination
K520 Ubiquitination
S587 Phosphorylation
S610 Phosphorylation
K620 Ubiquitination

Research Backgrounds

Function:

Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1. Regulates cell shape, spreading, and migration.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MPHOSPH6.

Restrictive clause

 

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