ARHGEF10 Antibody - #DF4430
(3)
| Product: | ARHGEF10 Antibody |
| Catalog: | DF4430 |
| Description: | Rabbit polyclonal antibody to ARHGEF10 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Prediction: | Sheep |
| Mol.Wt.: | 152 KD; 152kD(Calculated). |
| Uniprot: | O15013 |
| RRID: | AB_2836785 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Sheep(86%)
Clonality:
Polyclonal
Specificity:
ARHGEF10 Antibody detects endogenous levels of total ARHGEF10.
RRID:
AB_2836785
Cite Format: Affinity Biosciences Cat# DF4430, RRID:AB_2836785.
Cite Format: Affinity Biosciences Cat# DF4430, RRID:AB_2836785.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ARHGA_HUMAN; ARHGEF10; DKFZp686H0726; GEF10; KIAA0294; MGC131664; Rho guanine nucleotide exchange factor (GEF) 10; Rho guanine nucleotide exchange factor 10;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- O15013 ARHGA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRPPGFLSRAPSLNRAERGIWSCSMDQREPLPPAPAENEMKYDTNNNEEEEGEQFDFDSGDEIPEADRQAPSAPETGGAGASEAPAPTGGEDGAGAETTPVAEPTKLVLPMKVNPYSVIDITPFQEDQPPTPVPSAEEENVGLHVPCGYLVPVPCGYAVPSNLPLLLPAYSSPVIICATSLDEEAETPEVTEDRQPNSLSSEEPPTSEDQVGREDSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGDEGGNSSLEYGWSSSEFESYEEQSDSECKNGIPRSFLRSNHKKQLSHDLTRLKEHYEKKMRDLMASTVGVVEIQQLRQKHELKMQKLVKAAKDGTKDGLERTRAAVKRGRSFIRTKSLIAQDHRSSLEEEQNLFIDVDCKHPEAILTPMPEGLSQQQVVRRYILGSVVDSEKNYVDALKRILEQYEKPLSEMEPKVLSERKLKTVFYRVKEILQCHSLFQIALASRVSEWDSVEMIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKQEQEASPDRTTLYSLMMKPIQRFPQFILLLQDMLKNTSKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEVKQIAKAINERYLNKLLSSGSRYLIRSDDMIETVYNDRGEIVKTKERRVFMLNDVLMCATVSSRPSHDSRVMSSQRYLLKWSVPLGHVDAIEYGSSAGTGEHSRHLAVHPPESLAVVANAKPNKVYMGPGQLYQDLQNLLHDLNVIGQITQLIGNLKGNYQNLNQSVAHDWTSGLQRLILKKEDEIRAADCCRIQLQLPGKQDKSGRPTFFTAVFNTFTPAIKESWVNSLQMAKLALEEENHMGWFCVEDDGNHIKKEKHPLLVGHMPVMVAKQQEFKIECAAYNPEPYLNNESQPDSFSTAHGFLWIGSCTHQMGQIAIVSFQNSTPKVIECFNVESRILCMLYVPVEEKRREPGAPPDPETPAVRASDVPTICVGTEEGSISIYKSSQGSKKVRLQHFFTPEKSTVMSLACTSQSLYAGLVNGAVASYARAPDGSWDSEPQKVIKLGVLPVRSLLMMEDTLWAASGGQVFIISVETHAVEGQLEAHQEEGMVISHMAVSGVGIWIAFTSGSTLRLFHTETLKHLQDINIATPVHNMLPGHQRLSVTSLLVCHGLLMVGTSLGVLVALPVPRLQGIPKVTGRGMVSYHAHNSPVKFIVLATALHEKDKDKSRDSLAPGPEPQDEDQKDALPSGGAGSSLSQGDPDAAIWLGDSLGSMTQKSDLSSSSGSLSLSHGSSSLEHRSEDSTIYDLLKDPVSLRSKARRAKKAKASSALVVCGGQGHRRVHRKARQPHQEELAPTVMVWQIPLLNI
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Sheep
86
Pig
71
Horse
71
Bovine
71
Chicken
71
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15013 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S59 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| K441 | Sumoylation | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| Y629 | Phosphorylation | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| Y640 | Phosphorylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| K876 | Ubiquitination | Uniprot | |
| T980 | Phosphorylation | Uniprot | |
| Y1036 | Phosphorylation | Uniprot | |
| S1229 | Phosphorylation | Uniprot | |
| S1232 | Phosphorylation | Uniprot | |
| S1283 | Phosphorylation | Uniprot | |
| S1284 | Phosphorylation | Uniprot | |
| S1285 | Phosphorylation | Uniprot | |
| S1287 | Phosphorylation | Uniprot | |
| S1289 | Phosphorylation | Uniprot | |
| S1291 | Phosphorylation | Uniprot | |
| S1294 | Phosphorylation | Uniprot | |
| S1296 | Phosphorylation | Uniprot | |
| S1301 | Phosphorylation | Uniprot | |
| Y1307 | Phosphorylation | Uniprot | |
| K1311 | Ubiquitination | Uniprot | |
| S1315 | Phosphorylation | Uniprot | |
| Q1338 | Methylation | Uniprot |
Research Backgrounds
Function:
May play a role in developmental myelination of peripheral nerves.
PTMs:
Methylated at Gln-1338 by N6AMT1.
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