ARHGEF16 Antibody - #DF4433

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Product: ARHGEF16 Antibody
Catalog: DF4433
Description: Rabbit polyclonal antibody to ARHGEF16
Application: WB IF/ICC
Reactivity: Human
Prediction: Horse, Sheep, Dog
Mol.Wt.: 80 KD; 80kD(Calculated).
Uniprot: Q5VV41
RRID: AB_2836788

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(100%), Sheep(100%), Dog(83%)
Clonality:
Polyclonal
Specificity:
ARHGEF16 Antibody detects endogenous levels of total ARHGEF16.
RRID:
AB_2836788
Cite Format: Affinity Biosciences Cat# DF4433, RRID:AB_2836788.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGEF 16; ARHGEF16; ARHGG_HUMAN; Ephexin 4; Ephexin-4; EPHEXIN4; GEF16; NBR; Putative Neuroblastoma Protein; Rho guanine exchange factor (GEF) 16; Rho guanine nucleotide exchange factor (GEF) 16; Rho guanine nucleotide exchange factor 16;

Immunogens

Immunogen:
Uniprot:

Q5VV41(ARHGG_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGEF16(27237)
Sequence:
MAQRHSDSSLEEKLLGHRFHSELRLDAGGNPASGLPMVRGSPRVRDDAAFQPQVPAPPQPRPPGHEEPWPIVLSTESPAALKLGTQQLIPKSLAVASKAKTPARHQSFGAAVLSREAARRDPKLLPAPSFSLDDMDVDKDPGGMLRRNLRNQSYRAAMKGLGKPGGQGDAIQLSPKLQALAEEPSQPHTRSPAKNKKTLGRKRGHKGSFKDDPQLYQEIQERGLNTSQESDDDILDESSSPEGTQKVDATIVVKSYRPAQVTWSQLPEVVELGILDQLSTEERKRQEAMFEILTSEFSYQHSLSILVEEFLQSKELRATVTQMEHHHLFSNILDVLGASQRFFEDLEQRHKAQVLVEDISDILEEHAEKHFHPYIAYCSNEVYQQRTLQKLISSNAAFREALREIERRPACGGLPMLSFLILPMQRVTRLPLLMDTLCLKTQGHSERYKAASRALKAISKLVRQCNEGAHRMERMEQMYTLHTQLDFSKVKSLPLISASRWLLKRGELFLVEETGLFRKIASRPTCYLFLFNDVLVVTKKKSEESYMVQDYAQMNHIQVEKIEPSELPLPGGGNRSSSVPHPFQVTLLRNSEGRQEQLLLSSDSASDRARWIVALTHSERQWQGLSSKGDLPQVEITKAFFAKQADEVTLQQADVVLVLQQEDGWLYGERLRDGETGWFPEDFARFITSRVAVEGNVRRMERLRVETDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
83
Xenopus
63
Chicken
50
Pig
0
Bovine
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VV41 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
S8 Phosphorylation
S9 Phosphorylation
S21 Phosphorylation
S41 Phosphorylation
T101 Phosphorylation
S107 Phosphorylation
S129 Phosphorylation
K139 Ubiquitination
S153 Phosphorylation
K159 Methylation
S174 Phosphorylation
K176 Ubiquitination
S185 Phosphorylation
T189 Phosphorylation
S191 Phosphorylation
S208 Phosphorylation
K210 Ubiquitination
Y216 Phosphorylation
S227 Phosphorylation
S230 Phosphorylation
S240 Phosphorylation
S394 Phosphorylation
S418 Phosphorylation
S492 Phosphorylation
S499 Phosphorylation
S565 Phosphorylation
S576 Phosphorylation
S577 Phosphorylation
S578 Phosphorylation
K628 Ubiquitination
K638 Ubiquitination

Research Backgrounds

Function:

Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ELMO2, EPHA2, RAC1 and RHOG; mediates activation of RAC1 by EPHA2. Interacts with TAX1BP3 (via PDZ domain). May interact with CDC42; stimulated by HPV16 E6.

Family&Domains:

The PDZ-binding motif mediates interaction with TAX1BP3.

Restrictive clause

 

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