ARHGEF5 Antibody - #DF4435

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Product: ARHGEF5 Antibody
Catalog: DF4435
Description: Rabbit polyclonal antibody to ARHGEF5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 60 KD; 177kD(Calculated).
Uniprot: Q12774
RRID: AB_2836790

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
ARHGEF5 Antibody detects endogenous levels of total ARHGEF5.
RRID:
AB_2836790
Cite Format: Affinity Biosciences Cat# DF4435, RRID:AB_2836790.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHG5_HUMAN; ARHGEF 5; ARHGEF5; DKFZp686N1969; ephexin 3; Ephexin-3; GEF 5; GEF5; Guanine nucleotide regulatory protein TIM; Oncogene TIM; P60; p60 TIM; Rho guanine nucleotide exchange factor (GEF) 5; Rho guanine nucleotide exchange factor 5; TIM 1; TIM; TIM1; Transforming immortalized mammary oncogene;

Immunogens

Immunogen:
Uniprot:

Q12774(ARHG5_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGEF5(7984)
Expression:
Q12774 ARHG5_HUMAN:

Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624). Expressed at low levels in lung and heart (PubMed:14662653).

Sequence:
MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Dog
89
Pig
70
Sheep
58
Bovine
50
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12774 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S34 Phosphorylation
S57 Phosphorylation
T74 Phosphorylation
S77 Phosphorylation
S92 Phosphorylation
T100 Phosphorylation
Y182 Phosphorylation
S184 Phosphorylation
C186 S-Nitrosylation
S199 Phosphorylation
S340 Phosphorylation
S381 Phosphorylation
S395 Phosphorylation
S445 Phosphorylation
S450 Phosphorylation
S452 Phosphorylation
S464 Phosphorylation
S468 Phosphorylation
S474 Phosphorylation
T494 Phosphorylation
T514 Phosphorylation
S517 Phosphorylation
S521 Phosphorylation
Y579 Phosphorylation
S606 Phosphorylation
Y641 Phosphorylation
S642 Phosphorylation
S643 Phosphorylation
T645 Phosphorylation
S652 Phosphorylation
Y656 Phosphorylation
S657 Phosphorylation
T658 Phosphorylation
S660 Phosphorylation
S662 Phosphorylation
T735 Phosphorylation
S757 Phosphorylation
S793 Phosphorylation
T804 Phosphorylation
T816 Phosphorylation
S828 Phosphorylation
Y836 Phosphorylation
T849 Phosphorylation
R866 Methylation
S897 Phosphorylation
S908 Phosphorylation
S913 Phosphorylation
T972 Phosphorylation
S978 Phosphorylation
S983 Phosphorylation
S1011 Phosphorylation
S1036 Phosphorylation
S1044 Phosphorylation
K1060 Ubiquitination
S1083 Phosphorylation
S1087 Phosphorylation
S1092 Phosphorylation
Y1097 Phosphorylation
Y1100 Phosphorylation
K1107 Ubiquitination
S1117 Phosphorylation
S1119 Phosphorylation
T1121 Phosphorylation
S1125 Phosphorylation
S1126 Phosphorylation
S1136 Phosphorylation
Y1140 Phosphorylation
S1145 Phosphorylation
T1163 Phosphorylation
S1188 Phosphorylation
Y1189 Phosphorylation
Y1273 Phosphorylation
T1277 Phosphorylation
S1280 Phosphorylation
S1284 Phosphorylation
S1286 Phosphorylation
K1343 Ubiquitination
Y1370 Phosphorylation
S1403 Phosphorylation
S1405 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).

PTMs:

Activation of SRC induces tyrosine phosphorylation of ARHGEF5.

Subcellular Location:

Cytoplasm. Nucleus. Cell projection>Podosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver. Expressed at low levels in lung and heart.

Subunit Structure:

Interacts with SRC (By similarity). Forms a ternary complex with SRC and the PI3K 85 kDa subunit (By similarity). Interacts with and is activated by the heterodimer formed by GNB1 and GNG2 (By similarity). Interacts with ODAM (via C-terminus). Interacts with RHOA (By similarity).

Family&Domains:

The PH domain binds to phosphoinositides and is essential for podosome formation.

Restrictive clause

 

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For Research Use Only.
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