RPAP2 Antibody | Affinity Biosciences

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RPAP2 Antibody - Western blot analysis of extracts from various samples, using RPAP2 Antibody.

Product:	RPAP2 Antibody
Catalog:	DF4445
Description:	Rabbit polyclonal antibody to RPAP2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	70 KD; 70kD(Calculated).
Uniprot:	Q8IXW5
RRID:	AB_2836800

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

RPAP2 Antibody detects endogenous levels of total RPAP2.

RRID:

AB_2836800
Cite Format: Affinity Biosciences Cat# DF4445, RRID:AB_2836800.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2; RNA polymerase II-associated protein 2; RPAP2; RPAP2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q8IXW5(RPAP2_HUMAN) >>Visit HPA database.

Gene(ID):

RPAP2(79871)

Sequence:

Q8IXW5 RPAP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADFAGPSSAGRKAGAPRCSRKAAGTKQTSTLKQEDASKRKAELEAAVRKKIEFERKALHIVEQLLEENITEEFLMECGRFITPAHYSDVVDERSIVKLCGYPLCQKKLGIVPKQKYKISTKTNKVYDITERKSFCSNFCYQASKFFEAQIPKTPVWVREEERHPDFQLLKEEQSGHSGEEVQLCSKAIKTSDIDNPSHFEKQYESSSSSTHSDSSSDNEQDFVSSILPGNRPNSTNIRPQLHQKSIMKKKAGHKANSKHKDKEQTVVDVTEQLGDCKLDSQEKDATCELPLQKVNTQSSSNSTLPERLKASENSESEYSRSEITLVGISKKSAEHFKRKFAKSNQVSRSVSSSVQVCPEVGKRNLLKVLKETLIEWKTEETLRFLYGQNYASVCLKPEASLVKEELDEDDIISDPDSHFPAWRESQNSLDESLPFRGSGTAIKPLPSYENLKKETEKLNLRIREFYRGRYVLGEETTKSQDSEEHDSTFPLIDSSSQNQIRKRIVLEKLSKVLPGLLVPLQITLGDIYTQLKNLVRTFRLTNRNIIHKPAEWTLIAMVLLSLLTPILGIQKHSQEGMVFTRFLDTLLEELHLKNEDLESLTIIFRTSCLPE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Rabbit

Pig

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IXW5 As Substrate

Q8IXW5

Site	PTM Type	Source
A2	Acetylation	Uniprot
S9	Phosphorylation	Uniprot
T31	Phosphorylation	Uniprot
K125	Ubiquitination	Uniprot
Y127	Phosphorylation	Uniprot
T154	Phosphorylation	Uniprot
K171	Ubiquitination	Uniprot
S178	Phosphorylation	Uniprot
K190	Ubiquitination	Uniprot
K259	Ubiquitination	Uniprot
T297	Phosphorylation	Uniprot
K310	Ubiquitination	Uniprot
S315	Phosphorylation	Uniprot
Y319	Phosphorylation	Uniprot
K343	Ubiquitination	Uniprot
S352	Phosphorylation	Uniprot
K363	Ubiquitination	Uniprot
S414	Phosphorylation	Uniprot
S429	Phosphorylation	Uniprot
S433	Phosphorylation	Uniprot
K444	Ubiquitination	Uniprot
K453	Ubiquitination	Uniprot
S480	Phosphorylation	Uniprot
K509	Acetylation	Uniprot
K509	Ubiquitination	Uniprot

Research Backgrounds

Q8IXW5_RPAP2_HUMAN

Function:

Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner.

Subunit Structure:

Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD.

Family&Domains:

The RTR1-type zinc finger mediates interactions with RNA polymerase II complex subunits.

Belongs to the RPAP2 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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RPAP2 Antibody - #DF4445