SCFD1 Antibody - #DF4450

(2)  
Product: SCFD1 Antibody
Catalog: DF4450
Description: Rabbit polyclonal antibody to SCFD1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 75 KD; 72kD(Calculated).
Uniprot: Q8WVM8
RRID: AB_2836805

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
SCFD1 Antibody detects endogenous levels of total SCFD1.
RRID:
AB_2836805
Cite Format: Affinity Biosciences Cat# DF4450, RRID:AB_2836805.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C14orf163; Chromosome 14 open reading frame 163; RA410; Scfd1; SCFD1_HUMAN; Sec1 family domain containing 1; Sec1 family domain-containing protein 1; SLY1; SLY1 homolog; Sly1p; STXBP1L2; Syntaxin binding protein 1 like 2; Syntaxin-binding protein 1-like 2; Vesicle transport related protein;

Immunogens

Immunogen:
Uniprot:

Q8WVM8(SCFD1_HUMAN) >>Visit HPA database.

Gene(ID):
SCFD1(23256)
Sequence:
MAAAAAATAAAAASIRERQTVALKRMLNFNVPHIKNSTGEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAVYFVMPTEENIDRMCQDLRNQLYESYYLNFISAISRSKLEDIANAALAASAVTQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDTLGAGQFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESSGVENSPAGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDIISDPDAGTPEDKMRLFLIYYISTQQAPSEADLEQYKKALTDAGCNLNPLQYIKQWKAFTKMASAPASYGSTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRGNDSSVPRNKNPFQEAIVFVVGGGNYIEYQNLVDYIKGKQGKHILYGCSELFNATQFIKQLSQLGQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
88
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WVM8 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K24 Methylation
S57 Phosphorylation
K63 Ubiquitination
S113 Phosphorylation
Y114 Phosphorylation
Y115 Phosphorylation
K126 Ubiquitination
S297 Phosphorylation
S298 Phosphorylation
S303 Phosphorylation
K315 Ubiquitination
S316 Phosphorylation
K324 Ubiquitination
K330 Ubiquitination
S332 Phosphorylation
S360 Phosphorylation
S373 Phosphorylation
S376 Phosphorylation
T379 Phosphorylation
T383 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
K395 Ubiquitination
K417 Ubiquitination
K427 Ubiquitination
K431 Ubiquitination
K436 Ubiquitination
S443 Phosphorylation
K494 Ubiquitination
K501 Ubiquitination
Y509 Phosphorylation
K515 Ubiquitination
S528 Phosphorylation
K536 Acetylation
K536 Ubiquitination
K541 Ubiquitination
K558 Ubiquitination
K572 Ubiquitination
R575 Methylation
K642 Ubiquitination

Research Backgrounds

Function:

Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).

Subcellular Location:

Cytoplasm. Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with STX17. Interacts with STX5A. Interacts with the COG complex via COG4 (By similarity).

Family&Domains:

Belongs to the STXBP/unc-18/SEC1 family.

Restrictive clause

 

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For Research Use Only.
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