Product: SCAMP1 Antibody
Catalog: DF4453
Description: Rabbit polyclonal antibody to SCAMP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 32 KD; 38kD(Calculated).
Uniprot: O15126
RRID: AB_2836808

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(80%), Dog(100%), Chicken(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
SCAMP1 Antibody detects endogenous levels of total SCAMP1.
RRID:
AB_2836808
Cite Format: Affinity Biosciences Cat# DF4453, RRID:AB_2836808.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

SCAM1_HUMAN; SCAMP 1; SCAMP 37; SCAMP; SCAMP1; SCAMP37; Secretory carrier associated membrane protein 1; Secretory carrier membrane protein 1; Secretory carrier membrane protein; Secretory carrier-associated membrane protein 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15126 SCAM1_HUMAN:

Widely expressed, with highest expression in brain.

Sequence:
MSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELERKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWFCVDSARAVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFHNWGNCGWISSLTGLNQNIPVGIMMIIIAALFTASAVISLVMFKKVHGLYRTTGASFEKAQQEFATGVMSNKTVQTAAANAASTAASSAAQNAFKGNQI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Xenopus
86
Zebrafish
86
Horse
80
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15126 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
Y37 Phosphorylation
S41 Phosphorylation
T45 Phosphorylation
K52 Ubiquitination
T59 Phosphorylation
K65 Ubiquitination
T67 Phosphorylation
Y73 Phosphorylation
T74 Phosphorylation
K78 Ubiquitination
K89 Ubiquitination
K97 Ubiquitination
S112 Phosphorylation
K298 Ubiquitination
K311 Ubiquitination
K334 Acetylation
K334 Methylation
K334 Ubiquitination

Research Backgrounds

Function:

Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest expression in brain.

Subunit Structure:

Interacts with SYNRG and ITSN1 (By similarity). Interacts with SLC9A7.

Family&Domains:

Belongs to the SCAMP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.