NEK5 Antibody | Affinity Biosciences

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NEK5 Antibody - Western blot analysis of extracts from HepG2 cells using NEK5 antibody.

Product:	NEK5 Antibody
Catalog:	DF4466
Description:	Rabbit polyclonal antibody to NEK5
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	80 KD; 81kD(Calculated).
Uniprot:	Q6P3R8
RRID:	AB_2836821

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NEK5 Antibody detects endogenous levels of total NEK5.

RRID:

AB_2836821
Cite Format: Affinity Biosciences Cat# DF4466, RRID:AB_2836821.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC75495; NEK5; NEK5_HUMAN; Never in mitosis A-related kinase 5; NIMA (never in mitosis gene a) related kinase 5; NIMA related kinase 5; NimA related protein kinase 5; NimA-related protein kinase 5; OTTHUMP00000040896; Serine/threonine protein kinase Nek5; Serine/threonine-protein kinase Nek5;

Immunogens

Immunogen:

Uniprot:

Q6P3R8(NEK5_HUMAN) >>Visit HPA database.

Gene(ID):

NEK5(341676)

Sequence:

Q6P3R8 NEK5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDKYDVIKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSFQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYLTPEVIQEEFSHMLICRAGAPASRHAGKVVQKCKIQKVRFQGKCPPRSRISVPIKRNAILHRNEWRPPAGAQKARSIKMIERPKIAAVCGHYDYYYAQLDMLRRRAHKPSYHPIPQENTGVEDYGQETRHGPSPSQWPAEYLQRKFEAQQYKLKVEKQLGLRPSSAEPNYNQRQELRSNGEEPRFQELPFRKNEMKEQEYWKQLEEIRQQYHNDMKEIRKKMGREPEENSKISHKTYLVKKSNLPVHQDASEGEAPVQMEFRSCCPGWSAMARSWLTATSASQDIEKDLKQMRLQNTKESKNPEQKYKAKKGVKFEINLDKCISDENILQEEEAMDIPNETLTFEDGMKFKEYECVKEHGDYTDKAFEKLHCPEAGFSTQTVAAVGNRRQWDGGAPQTLLQMMAVADITSTCPTGPDSESVLSVSRQEGKTKDPYSPVLILM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Xenopus

100

Chicken

100

Rabbit

100

Sheep

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P3R8 As Substrate

Q6P3R8

Site	PTM Type	Enzyme	Source
Y477	Phosphorylation		Uniprot
K556	Acetylation		Uniprot

Research Backgrounds

Q6P3R8_NEK5_HUMAN

Family&Domains:

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NEK5 Antibody - #DF4466