TLK2 Antibody - #DF4478
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4478, RRID:AB_2836829.
Fold/Unfold
MGC44450; PKU alpha; PKU-alpha; Serine/threonine kinase; Serine/threonine protein kinase tousled like 2; Serine/threonine-protein kinase tousled-like 2; TLK 2; tlk2; TLK2_HUMAN; Tousled like kinase 2; Tousled-like kinase 2;
Immunogens
Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.
- Q86UE8 TLK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETPSSGNTELKDTAPALGAHSLLRLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86UE8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S686 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T695 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S753 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| S771 | Phosphorylation | Uniprot |
PTMs - Q86UE8 As Enzyme
Research Backgrounds
Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.
Phosphorylated at Ser-750, probably by CHEK1.
Nucleus. Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton.
Note: Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.
Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.
Monomer and heterodimer with TLK1. Interacts with ASF1A and ASF1B. Association with 14-3-3 proteins such as YWHAZ regulates subcellular location. May also interact with FEZ1/LZTS1 and FEZ2.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
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