ULK3 Antibody - #DF4479
| Product: | ULK3 Antibody |
| Catalog: | DF4479 |
| Description: | Rabbit polyclonal antibody to ULK3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 24 KD; 53kD(Calculated). |
| Uniprot: | Q6PHR2 |
| RRID: | AB_2836830 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4479, RRID:AB_2836830.
Fold/Unfold
DKFZP434C131; FLJ90566; Serine/threonine-protein kinase ULK3; Ulk3; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3;
Immunogens
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
- Q6PHR2 ULK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGIRHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSMGVILYEALFGQPPFASRSFSELEEKIRSNRVIELPLRPLLSRDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMPSGESLGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSSSNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6PHR2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R10 | Methylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Q6PHR2 (ULK3) | Uniprot |
| S305 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Q6PHR2 (ULK3) | Uniprot |
| S361 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Q6PHR2 (ULK3) | Uniprot |
| K441 | Ubiquitination | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Q6PHR2 (ULK3) | Uniprot |
PTMs - Q6PHR2 As Enzyme
Research Backgrounds
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.
Cytoplasm.
Note: Localizes to pre-autophagosomal structure during cellular senescence.
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
Interacts (via protein kinase domain) with SUFU.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
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