PPP4R2 Antibody - #DF4488
| Product: | PPP4R2 Antibody |
| Catalog: | DF4488 |
| Description: | Rabbit polyclonal antibody to PPP4R2 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Rabbit, Dog |
| Mol.Wt.: | 50 KD; 47kD(Calculated). |
| Uniprot: | Q9NY27 |
| RRID: | AB_2836839 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4488, RRID:AB_2836839.
Fold/Unfold
BE691708; C230060M08Rik; MGC131930; PP4R2; PP4R2_HUMAN; PPP4R2; protein phosphatase 4, regulatory subunit 2; Serine/threonine protein phosphatase 4 regulatory subunit 2; Serine/threonine-protein phosphatase 4 regulatory subunit 2;
Immunogens
A synthesized peptide derived from human PPP4R2, corresponding to a region within the internal amino acids.
- Q9NY27 PP4R2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGETEASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQEKESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSDCRETEELVGSNSSKTGEILSESSMENDDEATEVTDEPMEQD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus.
Note: Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2.
Widely expressed.
Belongs to the PPP4R2 family.
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