PPP4R2 Antibody - #DF4488
| Product: | PPP4R2 Antibody |
| Catalog: | DF4488 |
| Description: | Rabbit polyclonal antibody to PPP4R2 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Rabbit, Dog |
| Mol.Wt.: | 50 KD; 47kD(Calculated). |
| Uniprot: | Q9NY27 |
| RRID: | AB_2836839 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4488, RRID:AB_2836839.
Fold/Unfold
BE691708; C230060M08Rik; MGC131930; PP4R2; PP4R2_HUMAN; PPP4R2; protein phosphatase 4, regulatory subunit 2; Serine/threonine protein phosphatase 4 regulatory subunit 2; Serine/threonine-protein phosphatase 4 regulatory subunit 2;
Immunogens
- Q9NY27 PP4R2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGETEASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQEKESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSDCRETEELVGSNSSKTGEILSESSMENDDEATEVTDEPMEQD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NY27 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K246 | Acetylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K252 | Acetylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot |
Research Backgrounds
Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus.
Note: Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2.
Widely expressed.
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A and PPP4C-PPP4R2-PPP4R3B. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with DDX20/GEMIN3 and GEMIN4. Interacts with RPA2; this DNA damage-dependent interaction recruits PPP4C leading to RPA2 dephosphorylation.
Belongs to the PPP4R2 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.