SLC5A3 Antibody | Affinity Biosciences

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SLC5A3 Antibody - Western blot analysis of extracts from 293, using SLC5A3 Antibody.

Product:	SLC5A3 Antibody
Catalog:	DF4521
Description:	Rabbit polyclonal antibody to SLC5A3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	80 KD; 80kD(Calculated).
Uniprot:	P53794
RRID:	AB_2836872

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

SLC5A3 Antibody detects endogenous levels of total SLC5A3.

RRID:

AB_2836872
Cite Format: Affinity Biosciences Cat# DF4521, RRID:AB_2836872.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Na(+)/myo inositol cotransporter; Na(+)/myo-inositol cotransporter; SC5A3_HUMAN; SLC5A3; SMIT; SMIT2; sodium/myo inositol cotransporter 1; Sodium/myo inositol cotransporter; Sodium/myo-inositol cotransporter; solute carrier family 5 (inositol transporters), member 3; Solute carrier family 5 member 3;

Immunogens

Immunogen:

Uniprot:

P53794(SC5A3_HUMAN) >>Visit HPA database.

Sequence:

P53794 SC5A3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRAVLDTADIAIVALYFILVMCIGFFAMWKSNRSTVSGYFLAGRSMTWVTIGASLFVSNIGSEHFIGLAGSGAASGFAVGAWEFNALLLLQLLGWVFIPIYIRSGVYTMPEYLSKRFGGHRIQVYFAALSLILYIFTKLSVDLYSGALFIQESLGWNLYVSVILLIGMTALLTVTGGLVAVIYTDTLQALLMIIGALTLMIISIMEIGGFEEVKRRYMLASPDVTSILLTYNLSNTNSCNVSPKKEALKMLRNPTDEDVPWPGFILGQTPASVWYWCADQVIVQRVLAAKNIAHAKGSTLMAGFLKLLPMFIIVVPGMISRILFTDDIACINPEHCMLVCGSRAGCSNIAYPRLVMKLVPVGLRGLMMAVMIAALMSDLDSIFNSASTIFTLDVYKLIRKSASSRELMIVGRIFVAFMVVISIAWVPIIVEMQGGQMYLYIQEVADYLTPPVAALFLLAIFWKRCNEQGAFYGGMAGFVLGAVRLILAFAYRAPECDQPDNRPGFIKDIHYMYVATGLFWVTGLITVIVSLLTPPPTKEQIRTTTFWSKKNLVVKENCSPKEEPYQMQEKSILRCSENNETINHIIPNGKSEDSIKGLQPEDVNLLVTCREEGNPVASLGHSEAETPVDAYSNGQAALMGEKERKKETDDGGRYWKFIDWFCGFKSKSLSKRSLRDLMEEEAVCLQMLEETRQVKVILNIGLFAVCSLGIFMFVYFSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53794 As Substrate

P53794

Site	PTM Type	Source
Y16	Phosphorylation	Uniprot
Y39	Phosphorylation	Uniprot
K115	Ubiquitination	Uniprot
K249	Ubiquitination	Uniprot
K290	Ubiquitination	Uniprot
K296	Ubiquitination	Uniprot
S298	Phosphorylation	Uniprot
T299	Phosphorylation	Uniprot
S320	Phosphorylation	Uniprot
K549	Ubiquitination	Uniprot
K555	Ubiquitination	Uniprot
K570	Ubiquitination	Uniprot
K590	Ubiquitination	Uniprot
S591	Phosphorylation	Uniprot
S594	Phosphorylation	Uniprot
K596	Ubiquitination	Uniprot
T626	Phosphorylation	Uniprot
Y631	Phosphorylation	Uniprot
S632	Phosphorylation	Uniprot
K642	Ubiquitination	Uniprot
K671	Ubiquitination	Uniprot
S673	Phosphorylation	Uniprot

Research Backgrounds

P53794_SC5A3_HUMAN

Function:

Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SLC5A3 Antibody - #DF4521