SFRS15 Antibody - #DF4542
| Product: | SFRS15 Antibody |
| Catalog: | DF4542 |
| Description: | Rabbit polyclonal antibody to SFRS15 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 140 KD; 126kD(Calculated). |
| Uniprot: | O95104 |
| RRID: | AB_2836893 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4542, RRID:AB_2836893.
Fold/Unfold
arginine/serine-rich 15; CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and CTD-associated factor 4; SRA4;
Immunogens
- O95104 SCAF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAVNAFNQELFSLMDMKPPISRAKMILITKAAIKAIKLYKHVVQIVEKFIKKCKPEYKVPGLYVIDSIVRQSRHQFGTDKDVFGPRFSKNITATFQYLYLCPSEDKSKIVRVLNLWQKNGVFKIEIIQPLLDMAAGTSNAAPVAENVTNNEGSPPPPVKVSSEPPTQATPNSVPAVPQLPSSDAFAAVAQLFQTTQGQQLQQILQTFQQPPKPQSPALDNAVMAQVQAITAQLKTTPTQPSEQKAAFPPPEQKTAFDKKLLDRFDYDDEPEAVEESKKEDTTAVTTTAPAAAVPPAPTATVPAAAAPAAASPPPPQAPFGFPGDGMQQPAYTQHQNMDQFQPRMMGIQQDPMHHQVPLPPNGQMPGFGLLPTPPFPPMAQPVIPPTPPVQQPFQASFQAQNEPLTQKPHQQEMEVEQPCIQEVKRHMSDNRKSRSRSASRSPKRRRSRSGSRSRRSRHRRSRSRSRDRRRHSPRSRSQERRDREKERERRQKGLPQVKPETASVCSTTLWVGQLDKRTTQQDVASLLEEFGPIESINMIPPRGCAYIVMVHRQDAYRALQKLSRGNYKVNQKSIKIAWALNKGIKADYKQYWDVELGVTYIPWDKVKPEELESFCEGGMLDSDTLNPDWKGIPKKPENEVAQNGGAETSHTEPVSPIPKPLPVPVPPIPVPAPITVPPPQVPPHQPGPPVVGALQPPAFTPPLGIPPPGFGPGVPPPPPPPPFLRPGFNPMHLPPGFLPPGPPPPITPPVSIPPPHTPPISIPNSTIAGINEDTTKDLSIGNPIPTVVSGARGNAESGDSVKMYGSAVPPAAPTNLPTPPVTQPVSLLGTQGVAPGPVIGLQAPSTGLLGARPGLIPLQRPPGMPPPHLQRFPLMPPRPMPPHMMHRGPPPGPGGFAMPPPHGMKGPFPPHGPFVRPGGMPGLGGPGPGPGGPEDRDGRQQPPQQPQQQPQPQAPQQPQQQQQQQPPPSQQPPPTQQQPQQFRNDNRQQFNSGRDQERFGRRSFGNRVENDRERYGNRNDDRDNSNRDRREWGRRSPDRDRHRDLEERNRRSSGHRDRERDSRDRESRREKEEARGKEKPEVTDRAGGNKTVEPPISQVGNVDTASELEKGVSEAAVLKPSEELPAEATSSVEPEKDSGSAAEAPR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95104 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K49 | Acetylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| Y267 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| T652 | Phosphorylation | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| R888 | Methylation | Uniprot | |
| R917 | Methylation | Uniprot | |
| R937 | Methylation | Uniprot | |
| R940 | Methylation | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| S1037 | Phosphorylation | Uniprot | |
| S1054 | Phosphorylation | Uniprot | |
| S1068 | Phosphorylation | Uniprot | |
| R1086 | Methylation | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| S1131 | Phosphorylation | Uniprot | |
| S1141 | Phosphorylation | Uniprot |
Research Backgrounds
Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF8, also acts as a suppressor of transcriptional readthrough.
Nucleus.
Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5'.
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