Product: STMN4 Antibody
Catalog: DF4547
Description: Rabbit polyclonal antibody to STMN4
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 22 KD; 22kD(Calculated).
Uniprot: Q9H169
RRID: AB_2836898

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
STMN4 Antibody detects endogenous levels of total STMN4.
RRID:
AB_2836898
Cite Format: Affinity Biosciences Cat# DF4547, RRID:AB_2836898.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC111012; RB3; Stathmin like 4; Stathmin like protein RB3; Stathmin-4; Stathmin-like protein B3; Stmn4; STMN4_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human STMN4, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Sequence:
MTLAAYKEKMKELPLVSLFCSCFLADPLNKSSYKYEADTVDLNWCVISDMEVIELNKCTSGQSFEVILKPPSFDGVPEFNASLPRRRDPSLEEIQKKLEAAEERRKYQEAELLKHLAEKREHEREVIQKAIEENNNFIKMAKEKLAQKMESNKENREAHLAAMLERLQEKDKHAEEVRKNKELKEEASR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Exhibits microtubule-destabilizing activity.

Subcellular Location:

Golgi apparatus. Cell projection>Growth cone. Cell projection>Axon.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the stathmin family.

References

1). Copper overload impairs hematopoietic stem and progenitor cell proliferation via prompting HSF1/SP1 aggregation and the subsequently downregulating FOXM1-Cytoskeleton axis. iScience, 2023 (PubMed: 37009226) [IF=5.8]

Application: WB    Species: zebrafish    Sample: HSPCs

Figure 4 Cu overload destroys embryonic HSPC cytoskeleton (A) Schema for the experiments in (B) and (C). (B) Enriched GO terms for down-regulated DEGs in Cu-stressed HSPCs at 33 hpf. (C) Cytoskeleton protein immune-fluorescence for Cu-stressed HSPCs at 33 hpf. C1, C4, anti-β-tubulin staining; C2, C5, DAPI staining; C3, C6, merged; C7, the percentage of the Cu-stressed runx1GFP+ cells exhibiting destructive cytoskeleton; C8, calculation of the fluorescence intensity in each cell. (D) Mitotic malformation of HSPCs in the cytoskeleton gene morphants (tuba1a-MO, stmn4-MO, tubb5-MO, and tmsb2-MO together) at the 33 hpf (AGM) and 72 hpf (CHT), respectively. D1, D5, D10, D14, DAPI staining; D2, D6, D11, D15, anti-α-tubulin staining; D3, D7, D12, D16, anti-GFP staining; D4, D8, D13, D17, merged. At least 10 mitotic HSPCs in more than 10 embryos were observed for each group. D9, D18, calculation of the length of spindles in metaphase runx1GFP+ cells at 33 hpf and 72 hpf, respectively. (E) The protein level of Tuba1a (E1) and Stmn4 (E3) in tuba1a mutants and stmn4 mutants at 33 hpf, respectively. GAPDH was used as an internal control. (F) Phenotypes of tuba1a (F2, F5) mutants with a 4-bp deletion and stmn4 (F3, F6) mutants with an 8-bp deletion at 33 hpf and 120 hpf, respectively. (G) Expression of runx1 and cmyb in tuba1a mutants with or without Cu stresses at 72 hpf. G9, calculation of runx1 and cmyb expression in embryos from different groups. (H) Expression of runx1 and cmyb in stmn4 mutants with or without Cu stresses at 72 hpf. H9, calculation of runx1 and cmyb expression in embryos from different groups. F1-F6, G1-G8, H1-H8, lateral view, anterior to the left, and dorsal to the up. Data are mean ± SD. t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. NS, not significant. Scale bars, 1.2 μm (C1-C6), 2 μm (D1-D8, D10-D17), 100 μm (D1-D3, F1-F6), and 200 μm (G1-G8, H1-H8).

2). Deficiency of copper responsive gene stmn4 induces retinal developmental defects. Cell biology and toxicology, 2024 (PubMed: 38252267) [IF=5.3]

Application: WB    Species: Zebrafish    Sample:

Fig. 1 Stmn4 deficiency led to eye morphological defects. (A) Copper (Cu) stress induced obviously reduced expression of stmn4 in eyes during zebrafish embryogenesis (A1 − A4), cross-section of the eye (A5, A6) and the calculation of the relative expression levels of stmn4 (A7). (B) Western blot detection of Stmn4 protein levels in WT and stmn4−/− at 24 hpf and 48 hpf (B1 − B2), and the mRNA levels of stmn4 in WT and stmn4−/− at 24 and 48 hpf (B3), respectively. (C) Embryonic and eye development in stmn4−/− and WT embryos and larvae at different developmental stages (C1 − C12), and the quantification of the eye size in WT and stmn4−/− embryos and larvae (C13), respectively. (D) HE staining showed the difference in the eyes of WT and stmn4−/−embryos at 48 hpf (D1 − D4), and the calculation data (D5, D6). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. A1, A2, lateral view, anterior to the left, and dorsal to the up; A3, A4, head to the up, and dorsal to the down; C1 − C12, lateral view, anterior to the up, and dorsal to the right. Scale bar, 200 μm (A1 − A4), 100 μm (A5, A6, C1 − C12, D1, D2), 50 μm (D3, D4). ***P 

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For Research Use Only.
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