STK39 Antibody - #DF4548
| Product: | STK39 Antibody |
| Catalog: | DF4548 |
| Description: | Rabbit polyclonal antibody to STK39 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 60 KD; 59kD(Calculated). |
| Uniprot: | Q9UEW8 |
| RRID: | AB_2836899 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4548, RRID:AB_2836899.
Fold/Unfold
DCHT; DKFZp686K05124; OTTHUMP00000165175; PASK; Proline alanine rich STE20 related kinase; Serine threonine kinase 39 (STE20/SPS1 homolog yeast); Serine threonine kinase 39; Serine/threonine protein kinase 39; Serine/threonine-protein kinase 39; Small intestine SPAK like kinase; SPAK; Ste 20 related kinase; Ste-20-related kinase; Ste20 like protein kinase; STE20/SPS1 homolog; STE20/SPS1 related proline alanine rich protein kinase; STE20/SPS1-related proline-alanine-rich protein kinase; STK 39; Stk39; STK39_HUMAN;
Immunogens
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
- Q9UEW8 STK39_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UEW8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y63 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| T231 | Phosphorylation | Q9UEW8 (STK39) , Q96J92 (WNK4) , Q9H4A3 (WNK1) | Uniprot |
| T235 | Phosphorylation | Q9UEW8 (STK39) | Uniprot |
| Y273 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| K312 | Methylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K320 | Methylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| K324 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K349 | Acetylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Q9Y3S1 (WNK2) | Uniprot |
| S370 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Q9H4A3 (WNK1) , Q96J92 (WNK4) , Q9Y3S1 (WNK2) | Uniprot |
| S385 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| Y444 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| K512 | Acetylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot |
PTMs - Q9UEW8 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P55011 (SLC12A2) | T203 | Uniprot |
| P55011 (SLC12A2) | T207 | Uniprot |
| P55011 (SLC12A2) | T212 | Uniprot |
| P55017 (SLC12A3) | T46 | Uniprot |
| P55017 (SLC12A3) | T55 | Uniprot |
| P55017 (SLC12A3) | T60 | Uniprot |
| P55017 (SLC12A3) | S91 | Uniprot |
| Q13621 (SLC12A1) | T95 | Uniprot |
| Q13621 (SLC12A1) | T100 | Uniprot |
| Q13621 (SLC12A1) | T105 | Uniprot |
| Q9H2X9 (SLC12A5) | T6 | Uniprot |
| Q9H2X9 (SLC12A5) | T1032 | Uniprot |
| Q9UEW8 (STK39) | T231 | Uniprot |
| Q9UEW8 (STK39) | T235 | Uniprot |
| Q9UHW9 (SLC12A6) | S96 | Uniprot |
| Q9UHW9 (SLC12A6) | T160 | Uniprot |
| Q9UHW9 (SLC12A6) | T1048 | Uniprot |
| Q9UP95 (SLC12A4) | T5 | Uniprot |
| Q9UP95 (SLC12A4) | T983 | Uniprot |
| Q9Y666 (SLC12A7) | T973 | Uniprot |
| Q9Y666 (SLC12A7) | T980 | Uniprot |
| Q9Y6R1 (SLC4A4) | T49 | Uniprot |
| Q9Y6R1 (SLC4A4) | S65 | Uniprot |
Research Backgrounds
May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.
Phosphorylated at Ser-309 by PRKCQ. Autophosphorylation at Thr-231 positively regulates its activity (By similarity).
Cytoplasm. Nucleus.
Note: Nucleus when caspase-cleaved.
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
The phosphorylated form forms a complex with WNK2 (By similarity). Interacts with RELT (By similarity). Interacts with SORL1 (via cytosolic C-terminus).
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.