UBASH3A Antibody - #DF4550

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Product: UBASH3A Antibody
Catalog: DF4550
Description: Rabbit polyclonal antibody to UBASH3A
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 74 KD; 74kD(Calculated).
Uniprot: P57075
RRID: AB_2836901

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
UBASH3A Antibody detects endogenous levels of total UBASH3A.
RRID:
AB_2836901
Cite Format: Affinity Biosciences Cat# DF4550, RRID:AB_2836901.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cbl interacting protein 4; Cbl-interacting protein 4; CLIP4; STS 2; STS-2; Suppressor of T cell receptor signaling 2; Suppressor of T-cell receptor signaling 2; T cell ubiquitin ligand; T cell ubiquitin ligand protein; T-cell ubiquitin ligand 1; TULA; TULA-1; UBASH3A; ubiquitin associated and SH3 domain containing, A; Ubiquitin-associated and SH3 domain-containing protein A; UBS3A_HUMAN;

Immunogens

Immunogen:
Uniprot:

P57075(UBS3A_HUMAN) >>Visit HPA database.

Gene(ID):
UBASH3A(53347)
Expression:
P57075 UBS3A_HUMAN:

Highest expression of UBASH3A in tissues belonging to the immune system, including spleen, peripheral blood leukocytes, thymus and bone marrow.

Sequence:
MAAGETQLYAKVSNKLKSRSSPSLLEPLLAMGFPVHTALKALAATGRKTAEEALAWLHDHCNDPSLDDPIPQEYALFLCPTGPLLEKLQEFWRESKRQCAKNRAHEVFPHVTLCDFFTCEDQKVECLYEALKRAGDRLLGSFPTAVPLALHSSISYLGFFVSGSPADVIREFAMTFATEASLLAGTSVSRFWIFSQVPGHGPNLRLSNLTRASFVSHYILQKYCSVKPCTKQLHLTLAHKFYPHHQRTLEQLARAIPLGHSCQWTAALYSRDMRFVHYQTLRALFQYKPQNVDELTLSPGDYIFVDPTQQDEASEGWVIGISQRTGCRGFLPENYTDRASESDTWVKHRMYTFSLATDLNSRKDGEASSRCSGEFLPQTARSLSSLQALQATVARKSVLVVRHGERVDQIFGKAWLQQCSTPDGKYYRPDLNFPCSLPRRSRGIKDFENDPPLSSCGIFQSRIAGDALLDSGIRISSVFASPALRCVQTAKLILEELKLEKKIKIRVEPGIFEWTKWEAGKTTPTLMSLEELKEANFNIDTDYRPAFPLSALMPAESYQEYMDRCTASMVQIVNTCPQDTGVILIVSHGSTLDSCTRPLLGLPPRECGDFAQLVRKIPSLGMCFCEENKEEGKWELVNPPVKTLTHGANAAFNWRNWISGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Dog
82
Chicken
82
Rabbit
82
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P57075 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
K11 Ubiquitination
K15 Ubiquitination
S20 Phosphorylation
S21 Phosphorylation
S23 Phosphorylation
K48 Ubiquitination
K123 Acetylation
S213 Phosphorylation
K240 Ubiquitination
K347 Ubiquitination
S372 Phosphorylation
S382 Phosphorylation
S384 Phosphorylation
S385 Phosphorylation
K396 Ubiquitination
K413 Ubiquitination
S420 Phosphorylation
T421 Phosphorylation
K425 Ubiquitination
K445 Ubiquitination
S477 Phosphorylation
K491 Acetylation
K498 Ubiquitination
K501 Acetylation
K502 Acetylation
K516 Ubiquitination
K521 Ubiquitination
R564 Methylation

Research Backgrounds

Function:

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression of UBASH3A in tissues belonging to the immune system, including spleen, peripheral blood leukocytes, thymus and bone marrow.

Subunit Structure:

Homodimer or homooligomer. Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Interacts with dynamin.

Restrictive clause

 

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