ZIP7 Antibody - #DF4635

(2)   (5)  
Product: ZIP7 Antibody
Catalog: DF4635
Description: Rabbit polyclonal antibody to ZIP7
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50 KD; 50kD(Calculated).
Uniprot: Q92504
RRID: AB_2836926

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ZIP7 Antibody detects endogenous levels of total ZIP7.
RRID:
AB_2836926
Cite Format: Affinity Biosciences Cat# DF4635, RRID:AB_2836926.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D6S115E; D6S2244E; H2 KE4; Histidine rich membrane protein Ke4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene mouse, human homolog of; Really interesting new gene 5 protein; RING5; S39A7_HUMAN; SLC39A7; Solute carrier family 39 (zinc transporter), member 7; Solute carrier family 39 member 7; solute carrier family 39 zinc transporter member 7; Zinc transporter SLC39A7; ZIP7;

Immunogens

Immunogen:

A synthesized peptide derived from human ZIP7, corresponding to a region within the internal amino acids.

Uniprot:

Q92504(S39A7_HUMAN) >>Visit HPA database.

Gene(ID):
SLC39A7(7922)
Expression:
Q92504 S39A7_HUMAN:

Widely expressed.

Sequence:
MARGLGAPHWVAVGLLTWATLGLLVAGLGGHDDLHDDLQEDFHGHSHRHSHEDFHHGHSHAHGHGHTHESIWHGHTHDHDHGHSHEDLHHGHSHGYSHESLYHRGHGHDHEHSHGGYGESGAPGIKQDLDAVTLWAYALGATVLISAAPFFVLFLIPVESNSPRHRSLLQILLSFASGGLLGDAFLHLIPHALEPHSHHTLEQPGHGHSHSGQGPILSVGLWVLSGIVAFLVVEKFVRHVKGGHGHSHGHGHAHSHTRGSHGHGRQERSTKEKQSSEEEEKETRGVQKRRGGSTVPKDGPVRPQNAEEEKRGLDLRVSGYLNLAADLAHNFTDGLAIGASFRGGRGLGILTTMTVLLHEVPHEVGDFAILVQSGCSKKQAMRLQLLTAVGALAGTACALLTEGGAVGSEIAGGAGPGWVLPFTAGGFIYVATVSVLPELLREASPLQSLLEVLGLLGGVIMMVLIAHLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Sheep
91
Rabbit
91
Xenopus
64
Zebrafish
45
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Zinc transporter, that transports Zn(2+) from the endoplasmic reticulum/Golgi apparatus to the cytosol. Transport is stimulated by growth factors, such as EGF, and Ca(2+), as well as by exogenous Zn(2+).

PTMs:

Rapidly phosphorylated by CK2 following Zn(2+) treatment. This phosphorylation is required for efficient cytosolic Zn(2+) release.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus>cis-Golgi network membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily.

References

1). The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking. Autophagy, 2024 (PubMed: 37876250) [IF=14.6]
2). SYNJ2BP ameliorates intervertebral disc degeneration by facilitating mitochondria-associated endoplasmic reticulum membrane formation and mitochondrial Zn2+ homeostasis. Free radical biology & medicine, 2024 (PubMed: 38158052) [IF=7.1]

Application: WB    Species: human    Sample: NP cell

Fig. 2. MAM structure is essential for SLC39A7-NLRX1 complex formation and NP cell proliferation by maintaining mitochondrial Zn2+ homeostasis. (A) Confocal analysis of TOMM20 and CANX with IF staining in human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 10 μm. (B) MAM structure analysis by TEM in human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 1 μm. (C) Endogenous protein immunoprecipitated (IP) from NP cells isolated from health NP tissues with MFN2 knockdown or not, followed by western blotting, IP with anti-NLRX1 antibody. (D) Protein lysates extracted specially in mitochondria and cytoplasm of human NP cells isolated from health NP tissues with MFN2 knockdown or not, followed by western blotting. (E) Zinc distribution in primary human NP cells isolated from health NP tissues with MFN2 knockdown or not, detected by FluoZin™-3-AM labelling and confocal fluorescence microscope, scale bar: 10 μm. (F) Mitochondrial morphology analysis by fluorescence microscope with MitoTracker Red CMXRos label in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 5 μm. (G, H) Protein expressions of cell senescence indicators (TP53, CDKN2A) and extracellular matrix (COL2A1) in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, as determined by western blotting. (I-K) Cell senescence (SA-GLB1/β-gal staining) and cell proliferation (EdU incorporation) in primary human NP cells isolated from health NP tissues following the treatments of PBS or TBHP with MFN2 knockdown or not, scale bar: 100 μm (white light images), 200 μm (IF images). Data are represented as mean ± SD. *p 

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.