CA181 Antibody | Affinity Biosciences

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CA181 Antibody - Western blot analysis of extracts from Jurkat cells using CA181 antibody.

Product:	CA181 Antibody
Catalog:	DF4643
Description:	Rabbit polyclonal antibody to CA181
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	54 KD; 54kD(Calculated).
Uniprot:	Q9NWK9
RRID:	AB_2836934

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

CA181 Antibody detects endogenous levels of total CA181.

RRID:

AB_2836934
Cite Format: Affinity Biosciences Cat# DF4643, RRID:AB_2836934.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BCD1; BCD1_HUMAN; Box C/D snoRNA essential 1 homolog; Box C/D snoRNA protein 1; Chromosome 1 open reading frame 181; FLJ20729; FLJ20760; HIT type zinc finger containing protein C1orf181; Hypothetical protein LOC54680; MGC131963; NY BR 75; NYBR75; Serologically defined breast cancer antigen NY BR 75; Serologically defined breast cancer antigen NY-BR-75; Zinc finger HIT domain-containing protein 6; Zinc finger, HIT type 6; Zinc finger, HIT type containing 6; ZNHIT6;

Immunogens

Immunogen:

Uniprot:

Q9NWK9(BCD1_HUMAN) >>Visit HPA database.

Gene(ID):

ZNHIT6(54680)

Sequence:

Q9NWK9 BCD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEFAAENEGKSGGGLHSVAEGVRLSPEPGREGVRDLAGAEEFGGGEEGTGLTGIKEIGDGEEGSGQRPEEIPMDLTVVKQEIIDWPGTEGRLAGQWVEQEVEDRPEVKDENAGVLEVKQETDSSLVVKEAKVGEPEVKEEKVKEEVMDWSEVKEEKDNLEIKQEEKFVGQCIKEELMHGECVKEEKDFLKKEIVDDTKVKEEPPINHPVGCKRKLAMSRCETCGTEEAKYRCPRCMRYSCSLPCVKKHKAELTCNGVRDKTAYISIQQFTEMNLLSDYRFLEDVARTADHISRDAFLKRPISNKYMYFMKNRARRQGINLKLLPNGFTKRKENSTFFDKKKQQFCWHVKLQFPQSQAEYIEKRVPDDKTINEILKPYIDPEKSDPVIRQRLKAYIRSQTGVQILMKIEYMQQNLVRYYELDPYKSLLDNLRNKVIIEYPTLHVVLKGSNNDMKVLHQVKSESTKNVGNEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NWK9 As Substrate

Q9NWK9

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S25	Phosphorylation	Uniprot
T49	Phosphorylation	Uniprot
T52	Phosphorylation	Uniprot
K108	Sumoylation	Uniprot
K118	Sumoylation	Uniprot
T121	Phosphorylation	Uniprot
S123	Phosphorylation	Uniprot
K138	Sumoylation	Uniprot
K143	Sumoylation	Uniprot
K153	Sumoylation	Uniprot
K162	Sumoylation	Uniprot
K173	Sumoylation	Uniprot
T197	Phosphorylation	Uniprot
K200	Sumoylation	Uniprot
K212	Ubiquitination	Uniprot
K229	Ubiquitination	Uniprot
K249	Sumoylation	Uniprot
K249	Ubiquitination	Uniprot
K298	Ubiquitination	Uniprot
K310	Ubiquitination	Uniprot
K321	Ubiquitination	Uniprot
K329	Methylation	Uniprot
K329	Ubiquitination	Uniprot
K331	Ubiquitination	Uniprot
K340	Ubiquitination	Uniprot
K341	Ubiquitination	Uniprot
K362	Ubiquitination	Uniprot
K368	Ubiquitination	Uniprot
K375	Ubiquitination	Uniprot
K382	Ubiquitination	Uniprot
Y423	Phosphorylation	Uniprot
K424	Ubiquitination	Uniprot
S425	Phosphorylation	Uniprot
K433	Ubiquitination	Uniprot
K453	Ubiquitination	Uniprot
K459	Sumoylation	Uniprot
K459	Ubiquitination	Uniprot
K464	Ubiquitination	Uniprot

Research Backgrounds

Q9NWK9_BCD1_HUMAN

Function:

Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.

Subunit Structure:

Interacts with FBL, SNU13, NOP58, NUFIP1, RUVBL1, RUVBL2 and TAF9. Interacts (via HIT-type zinc finger) with the RUVBL1/RUVBL2 complex in the presence of ADP.

Family&Domains:

Belongs to the BCD1 family.

Restrictive clause

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For Research Use Only.
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CA181 Antibody - #DF4643