MTIF3 Antibody | Affinity Biosciences

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MTIF3 Antibody - Western blot analysis of extracts from HuvEC cells, using MTIF3 antibody.

Product:	MTIF3 Antibody
Catalog:	DF4579
Description:	Rabbit polyclonal antibody to MTIF3
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	30 KD; 32kD(Calculated).
Uniprot:	Q9H2K0
RRID:	AB_2836943

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

MTIF3 Antibody detects endogenous levels of total MTIF3.

RRID:

AB_2836943
Cite Format: Affinity Biosciences Cat# DF4579, RRID:AB_2836943.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DC38; IF 3; IF 3Mt; IF-3(Mt); IF-3Mt; IF3; IF3(mt); IF3M_HUMAN; IF3mt; mitochondrial; Mt; MTIF3; Translation initiation factor IF 3, mitochondrial precursor; Translation initiation factor IF-3;

Immunogens

Immunogen:

Uniprot:

Q9H2K0(IF3M_HUMAN) >>Visit HPA database.

Gene(ID):

MTIF3(219402)

Sequence:

Q9H2K0 IF3M_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALFLKRLTLQTVKSENSCIRCFGKHILQKTAPAQLSPIASAPRLSFLIHAKAFSTAEDTQNEGKKTKKNKTAFSNVGRKISQRVIHLFDEKGNDLGNMHRANVIRLMDERDLRLVQRNTSTEPAEYQLMTGLQILQERQRLREMEKANPKTGPTLRKELILSSNIGQHDLDTKTKQIQQWIKKKHLVQITIKKGKNVDVSENEMEEIFHQILQTMPGIATFSSRPQAVQGGKALMCVLRAFSKNEEKAYKETQETQERDTLNKDHGNDKESNVLHQ

PTMs - Q9H2K0 As Substrate

Q9H2K0

Site	PTM Type	Enzyme	Source
T61	Phosphorylation		Uniprot

Research Backgrounds

Q9H2K0_IF3M_HUMAN

Function:

IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.

Subcellular Location:

Mitochondrion.

Family&Domains:

Belongs to the IF-3 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MTIF3 Antibody - #DF4579