TSEN54 Antibody | Affinity Biosciences

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TSEN54 Antibody - Western blot analysis of extracts from 3T3 cells using TSEN54 antibody.

Product:	TSEN54 Antibody
Catalog:	DF4581
Description:	Rabbit polyclonal antibody to TSEN54
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine
Mol.Wt.:	59 KD; 59kD(Calculated).
Uniprot:	Q7Z6J9
RRID:	AB_2836945

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(80%)

Clonality:

Polyclonal

Specificity:

TSEN54 Antibody detects endogenous levels of total TSEN54.

RRID:

AB_2836945
Cite Format: Affinity Biosciences Cat# DF4581, RRID:AB_2836945.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HsSen54; PCH2A; PCH4; sen54; SEN54 homolog; SEN54_HUMAN; SEN54L; tRNA-intron endonuclease SEN54; tRNA-splicing endonuclease subunit SEN54; Tsen54; TSEN54 tRNA splicing endonuclease subunit;

Immunogens

Immunogen:

Uniprot:

Q7Z6J9(SEN54_HUMAN) >>Visit HPA database.

Gene(ID):

TSEN54(283989)

Sequence:

Q7Z6J9 SEN54_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPEPEPAAVEVPAGRVLSARELFAARSRSQKLPQRSHGPKDFLPDGSAAQAERLRRCREELWQLLAEQRVERLGSLVAAEWRPEEGFVELKSPAGKFWQTMGFSEQGRQRLHPEEALYLLECGSIHLFHQDLPLSIQEAYQLLLTDHTVTFLQYQVFSHLKRLGYVVRRFQPSSVLSPYERQLNLDASVQHLEDGDGKRKRSSSSPRSINKKAKALDNSLQPKSLAASSPPPCSQPSQCPEEKPQESSPMKGPGGPFQLLGSLGPSPGPAREGVGCSWESGRAENGVTGAGKRRWNFEQISFPNMASDSRHTLLRAPAPELLPANVAGRETDAESWCQKLNQRKEKLSRREREHHAEAAQFQEDVNADPEVQRCSSWREYKELLQRRQVQRSQRRAPHLWGQPVTPLLSPGQASSPAVVLQHISVLQTTHLPDGGARLLEKSGGLEIIFDVYQADAVATFRKNNPGKPYARMCISGFDEPVPDLCSLKRLSYQSGDVPLIFALVDHGDISFYSFRDFTLPQDVGH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Rabbit

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z6J9 As Substrate

Q7Z6J9

Site	PTM Type	Source
M1	Acetylation	Uniprot
S76	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
K97	Ubiquitination	Uniprot
S178	Phosphorylation	Uniprot
Y180	Phosphorylation	Uniprot
K199	Acetylation	Uniprot
S203	Phosphorylation	Uniprot
S204	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
S225	Phosphorylation	Uniprot
S229	Phosphorylation	Uniprot
S230	Phosphorylation	Uniprot
S235	Phosphorylation	Uniprot
S249	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
K293	Acetylation	Uniprot
R316	Methylation	Uniprot
K340	Ubiquitination	Uniprot
K382	Ubiquitination	Uniprot
S443	Phosphorylation	Uniprot
K489	Ubiquitination	Uniprot

Research Backgrounds

Q7Z6J9_SEN54_HUMAN

Function:

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: May be transiently localized in the nucleolus.

Subunit Structure:

tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2. Also belongs to a complex containing isoform 2 of SEN2.

Family&Domains:

Belongs to the SEN54 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TSEN54 Antibody - #DF4581