SGK269 Antibody - #DF4583

(2)  
Product: SGK269 Antibody
Catalog: DF4583
Description: Rabbit polyclonal antibody to SGK269
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 193 KD; 193kD(Calculated).
Uniprot: Q9H792
RRID: AB_2836947

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
SGK269 Antibody detects endogenous levels of total SGK269.
RRID:
AB_2836947
Cite Format: Affinity Biosciences Cat# DF4583, RRID:AB_2836947.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ21140; FLJ34483; KIAA2002; NKF3 kinase family member; PEAK1; PEAK1_HUMAN; pseudopodium enriched atypical kinase; Pseudopodium-enriched atypical kinase 1; SGK269; Sugen kinase 269; Tyrosine-protein kinase SgK269;

Immunogens

Immunogen:
Uniprot:

Q9H792(PEAK1_HUMAN) >>Visit HPA database.

Gene(ID):
PEAK1(79834)
Sequence:
MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNVKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMIVADGQSICGELSIQEHCENKPVIIGWNRNRAALSQKPLNNNNEDDEGISHVPKPYGNNDSAKKMSDNNNGLTEVLKEIAGLDTAPQIRGNETNSRETFLGRINDCYKRSLERKLPPSCMIGGIKETQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLFSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTIPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGQGSIQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGLSPGNPGDSKDMKEIEPNYESPSSNNQDKDSSQASKSSIKVPETHKAVLALRLEEKDGKIAVQTEKEESKASTDVAGQAVTINLVPTEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGPVNSPKTKSSSSTPNSPVTSSSLTPGQISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELITSGTGPNVPPRKNCHKSAPTSPTATNISSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKVPDNTTSKTTDCLQTKGFSNSTEHKRGSVAQKVQEFNNCLNRGQSSPQRSYSSSHSSPAKIQRATQEPVAKIEGTQESQMVGSSSTREKASTVLSQIVASIQPPQSPPETPQSGPKACSVEELYAIPPDADVAKSTPKSTPVRPKSLFTSQPSGEAEAPQTTDSPTTKVQKDPSIKPVTPSPSKLVTSPQSEPPAPFPPPRSTSSPYHAGNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKEREKGKLVGLDGTVIHMLPPPPVQRHHWFTEAKGESSEKPAIVFMYRCDPAQGQLSVDQSKARTDQAAVMEKGRAENALLQDSEKKRSHSSPSQIPKKILSHMTHEVTEDFSPRDPRTVVGKQDGRGCTSVTTALSLPELEREDGKEDISDPMDPNPCSATYSNLGQSRAAMIPPKQPRQPKGAVDDAIAFGGKTDQEAPNASQPTPPPLPKKMIIRANTEPISKDLQKSMESSLCVMANPTYDIDPNWDASSAGSSISYELKGLDIESYDSLERPLRKERPVPSAANSISSLTTLSIKDRFSNSMESLSSRRGPSCRQGRGIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAVRQSLAVHFNIQQDCGHFLAEVPNRLLPWEDPDDPEKDEDDMEETEEDAKGETDGKNPKPCSEAASSQKENQGVMSKKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDLYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGTAQGFGPAEPSPTSSYPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEREYTRADLPRIPFRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTACPSLVQRNTLLQNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQYLAFATTDSLSCIVKILQHR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Bovine
91
Sheep
91
Dog
91
Chicken
82
Xenopus
70
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H792 As Substrate

Site PTM Type Enzyme
S212 Phosphorylation
S214 Phosphorylation
T279 Phosphorylation
S281 Phosphorylation
T293 Phosphorylation
S359 Phosphorylation
S370 Phosphorylation
S377 Phosphorylation
Y387 Phosphorylation
S389 Phosphorylation
Y462 Phosphorylation
Y475 Phosphorylation
S495 Phosphorylation
S503 Phosphorylation
T504 Phosphorylation
S507 Phosphorylation
T510 Phosphorylation
T515 Phosphorylation
S527 Phosphorylation
Y531 Phosphorylation
T539 Phosphorylation
S540 Phosphorylation
K563 Acetylation
K567 Acetylation
S568 Phosphorylation
T571 Phosphorylation
S572 Phosphorylation
T574 Phosphorylation
T576 Phosphorylation
K581 Acetylation
S587 Phosphorylation
Y598 Phosphorylation
T615 Phosphorylation
Y616 Phosphorylation
Y635 Phosphorylation P07948 (LYN)
Y641 Phosphorylation
S643 Phosphorylation
S648 Phosphorylation
S652 Phosphorylation
T657 Phosphorylation
Y665 Phosphorylation
S725 Phosphorylation
S729 Phosphorylation
S730 Phosphorylation
T759 Phosphorylation
S779 Phosphorylation
T783 Phosphorylation
S786 Phosphorylation
S792 Phosphorylation
Y797 Phosphorylation
S812 Phosphorylation
T813 Phosphorylation
S819 Phosphorylation
T822 Phosphorylation
S826 Phosphorylation
T834 Phosphorylation
S837 Phosphorylation
S847 Phosphorylation
T852 Phosphorylation
S854 Phosphorylation
S856 Phosphorylation
T860 Phosphorylation
S861 Phosphorylation
S864 Phosphorylation
T876 Phosphorylation
S877 Phosphorylation
S878 Phosphorylation
Y880 Phosphorylation
T897 Phosphorylation
S898 Phosphorylation
T900 Phosphorylation
K921 Acetylation
S927 Phosphorylation
T937 Phosphorylation
T975 Phosphorylation
S981 Phosphorylation
S1001 Phosphorylation
S1028 Phosphorylation
S1035 Phosphorylation
S1036 Phosphorylation
S1038 Phosphorylation
S1046 Phosphorylation
S1057 Phosphorylation
T1074 Phosphorylation
T1077 Phosphorylation
T1078 Phosphorylation
S1104 Phosphorylation
T1106 Phosphorylation
Y1107 Phosphorylation
T1151 Phosphorylation
T1165 Phosphorylation
Y1188 Phosphorylation
S1214 Phosphorylation
Y1215 Phosphorylation
S1217 Phosphorylation
S1234 Phosphorylation
S1248 Phosphorylation
S1250 Phosphorylation
S1253 Phosphorylation
S1256 Phosphorylation
S1292 Phosphorylation
S1347 Phosphorylation
Y1348 Phosphorylation
Y1371 Phosphorylation
Y1372 Phosphorylation
S1374 Phosphorylation
S1563 Phosphorylation
T1583 Phosphorylation
Y1587 Phosphorylation
S1657 Phosphorylation

Research Backgrounds

Function:

Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.

PTMs:

Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Phosphorylation at Tyr-665 by a Src family kinase controls subcellular localization to focal adhesion and focal adhesion dynamics. Phosphorylation at Tyr-1188 is essential for binding to SHC1.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion.
Note: Colocalizes with F-actin in serum-rich medium (PubMed:20534451). Actin colocalization is reduced during serum starvation (PubMed:20534451).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with BCAR1 and CRK. Interacts with PRAG1. Interacts (when phosphorylated at Tyr-1188) with SHC1 (via PID domain). Found in a complex with PPP1CA, PPP1CC, SHC1 and PEAK1.

Family&Domains:

The dimerization region encompasses helices both from the N- and C-terminal of the protein kinase domain.

Belongs to the protein kinase superfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.