USP11 Antibody - #DF4586

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Product: USP11 Antibody
Catalog: DF4586
Description: Rabbit polyclonal antibody to USP11
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 109 KD; 110kD(Calculated).
Uniprot: P51784
RRID: AB_2836950

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(85%)
Clonality:
Polyclonal
Specificity:
USP11 Antibody detects endogenous levels of total USP11.
RRID:
AB_2836950
Cite Format: Affinity Biosciences Cat# DF4586, RRID:AB_2836950.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deubiquitinating enzyme 11; Ubiquitin carboxyl-terminal hydrolase 11; Ubiquitin carboxyl-terminal hydrolase X linked; ubiquitin specific peptidase 11; Ubiquitin specific protease 11; Ubiquitin thiolesterase 11; Ubiquitin-specific-processing protease 11; UBP11_HUMAN; UHX1; USP11;

Immunogens

Immunogen:
Uniprot:

P51784(UBP11_HUMAN) >>Visit HPA database.

Gene(ID):
USP11(8237)
Sequence:
MAVAPRLFGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
85
Pig
69
Horse
69
Bovine
69
Sheep
69
Zebrafish
38
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51784 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
S33 Phosphorylation
K107 Ubiquitination
K182 Ubiquitination
K191 Ubiquitination
Y195 Phosphorylation
R241 Methylation
K245 Acetylation
K245 Ubiquitination
T268 Phosphorylation
T276 Phosphorylation
K303 Sumoylation
K355 Ubiquitination
K367 Ubiquitination
K383 Methylation
K383 Ubiquitination
Y396 Phosphorylation
K422 Ubiquitination
K447 Ubiquitination
S452 Phosphorylation
K493 Ubiquitination
K531 Ubiquitination
S584 Phosphorylation
Y593 Phosphorylation
S606 Phosphorylation
Y607 Phosphorylation
Y608 Phosphorylation
T644 Phosphorylation
S648 Phosphorylation
K664 Ubiquitination
S671 Phosphorylation
T672 Phosphorylation
S675 Phosphorylation
R677 Methylation
K714 Ubiquitination
T731 Phosphorylation
S733 Phosphorylation
Y742 Phosphorylation
K752 Ubiquitination
K766 Ubiquitination
K775 Ubiquitination
K806 Ubiquitination
K813 Ubiquitination
K835 Ubiquitination
K840 Ubiquitination
K871 Ubiquitination
K896 Ubiquitination
K920 Ubiquitination
Y923 Phosphorylation
S938 Phosphorylation
S942 Phosphorylation
S943 Phosphorylation
S948 Phosphorylation
S952 Phosphorylation
S953 Phosphorylation
S956 Phosphorylation
S957 Phosphorylation

Research Backgrounds

Function:

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro). Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: Predominantly nuclear (PubMed:12084015, PubMed:15314155). Associates with chromatin (PubMed:20601937, PubMed:20233726).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Interacts with RANBP9/RANBPM. Interacts with BRCA2. Interacts with CHUK/IKKA. Interacts with NFKBIA. Associated component of the Polycomb group (PcG) multiprotein PRC1-like complex.

(Microbial infection) Interacts with papilloma virus protein 16E7.

Family&Domains:

Belongs to the peptidase C19 family.

Restrictive clause

 

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