USP44 Antibody - #DF4594
| Product: | USP44 Antibody |
| Catalog: | DF4594 |
| Description: | Rabbit polyclonal antibody to USP44 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 81 KD; 81kD(Calculated). |
| Uniprot: | Q9H0E7 |
| RRID: | AB_2836958 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4594, RRID:AB_2836958.
Fold/Unfold
Deubiquitinating enzyme 44; E430004F17Rik; Ubiquitin carboxyl-terminal hydrolase 44; ubiquitin specific peptidase 44; Ubiquitin thiolesterase 44; Ubiquitin-specific-processing protease 44; UBP44_HUMAN; USP44;
Immunogens
Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia.
- Q9H0E7 UBP44_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKHFQESSHPVALEVNEMYVFCYLCDDYVLNDNTTGDLKLLRRTLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQSSLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNMEPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPNEDADTSSNEILS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0E7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T34 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K667 | Acetylation | Uniprot |
Research Backgrounds
Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.
Dephosphorylated by CTDP1.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.
Nucleus.
Note: Peaks in interphase, with relatively low levels maintained throughout mitosis.
Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia.
Belongs to the peptidase C19 family. USP44 subfamily.
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