UBAP2L Antibody - #DF4599
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| Product: | UBAP2L Antibody |
| Catalog: | DF4599 |
| Description: | Rabbit polyclonal antibody to UBAP2L |
| Application: | WB IHC IF/ICC |
| Cited expt.: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 115 KD; 115kD(Calculated). |
| Uniprot: | Q14157 |
| RRID: | AB_2836963 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
UBAP2L Antibody detects endogenous levels of total UBAP2L.
RRID:
AB_2836963
Cite Format: Affinity Biosciences Cat# DF4599, RRID:AB_2836963.
Cite Format: Affinity Biosciences Cat# DF4599, RRID:AB_2836963.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ42300; KIAA0144; NICE 4; NICE4; Protein NICE-4; Protein NICE4; Ubap2l; Ubiquitin associated protein 2 like; Ubiquitin-associated protein 2-like; UBP2L_HUMAN;
Immunogens
Immunogen:
A synthesized peptide derived from human UBAP2L, corresponding to a region within the internal amino acids.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q14157 UBP2L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILHHHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Function:
Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs).
PTMs:
Acetylated.
Tissue Specificity:
Ubiquitous.
References
1). Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells. Bioengineered, 2021
(PubMed: 34823423)
[IF=4.2]
Application: WB Species: Human Sample: gastric cancer cells
Figure 3.
Silencing of UBAP2L repressed proliferation and promoted apoptosis of gastric cancer cells
a and b. The mRNA and protein levels of UBAP2L in normal human gastric mucosa cells and gastric cancer cells were determined by real-time PCR and western blot, respectively. c and d. The mRNA and protein levels of UBAP2L in AGS and NCI-N87 cells were detected after transfection of siRNA targeting UBAP2L. e and f. CCK-8 assay was performed for measurement of cell viability in UBAP2L-silenced AGS and NCI-N87 cells. g-i. The cell cycle distribution of AGS and NCI-N87 cells was examined by flow cytometry. j. The proliferation index (PI) of AGS and NCI-N87 cells were calculated based on the cell numbers in each phase of the cell cycle (PI = (cell numbers in G2/M and S phases)/cell numbers in G1/G0 phase). k and l. The apoptosis of AGS and NCI-N87 cells were detected by flow cytometry. (*p < 0.05, **p < 0.01, ***p < 0.001, compared with siRNA NC)
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