Product: UEVLD Antibody
Catalog: DF4602
Description: Rabbit polyclonal antibody to UEVLD
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Rat, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 52 KD; 52kD(Calculated).
Uniprot: Q8IX04
RRID: AB_2836966

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rat(%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
UEVLD Antibody detects endogenous levels of total UEVLD.
RRID:
AB_2836966
Cite Format: Affinity Biosciences Cat# DF4602, RRID:AB_2836966.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

8430408E05Rik; ATTP; EV and lactate malate dehydrogenase domain containing protein; EV and lactate/malate dehydrogenase domain-containing protein; FLJ11068; signaling molecule ATTP; ubiquitin conjugating enzyme E2 like; Ubiquitin conjugating enzyme E2 variant 3; Ubiquitin E2 variant and lactate/malate dehydrogenase domain containing protein; Ubiquitin-conjugating enzyme E2 variant 3; UEV 3; UEV and lactate malate dehyrogenase domains; UEV-3; UEV2 and LDH domains containing protein; UEV3; uevld; UEVLD_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8IX04 UEVLD_HUMAN:

Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.

Sequence:
MEFDCEGLRRLLGKYKFRDLTVEELRNVNVFFPHFKYSMDTYVFKDSSQKDLLNFTGTIPVMYQGNTYNIPIRFWILDSHPFAPPICFLKPTANMGILVGKHVDAQGRIYLPYLQNWSHPKSVIVGLIKEMIAKFQEELPMYSLSSSDEARQVDLLAYIAKITEGVSDTNSKSWANHENKTVNKITVVGGGELGIACTLAISAKGIADRLVLLDLSEGTKGATMDLEIFNLPNVEISKDLSASAHSKVVIFTVNSLGSSQSYLDVVQSNVDMFRALVPALGHYSQHSVLLVASQPVEIMTYVTWKLSTFPANRVIGIGCNLDSQRLQYIITNVLKAQTSGKEVWVIGEQGEDKVLTWSGQEEVVSHTSQVQLSNRAMELLRVKGQRSWSVGLSVADMVDSIVNNKKKVHSVSALAKGYYDINSEVFLSLPCILGTNGVSEVIKTTLKEDTVTEKLQSSASSIHSLQQQLKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
63
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IX04 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
K172 Ubiquitination
K180 Ubiquitination
T181 Phosphorylation
T186 Phosphorylation
T198 Phosphorylation
S323 Phosphorylation
K341 Ubiquitination
K407 Ubiquitination
K454 Ubiquitination

Research Backgrounds

Function:

Possible negative regulator of polyubiquitination.

Tissue Specificity:

Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.

Subunit Structure:

Homodimer.

Family&Domains:

In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.

In the C-terminal section; belongs to the LDH/MDH superfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.