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Product Info

ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1610]
EphB2 antibody detects endogenous levels of total EphB2.
Cite Format: Affinity Biosciences Cat# BF0703, RRID:AB_2833634.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


cek5; Developmentally regulated EPH related tyrosine kinase; DRT; EK5; ELK related protein tyrosine kinase; Eph receptor B2; EPH tyrosine kinase 3; EPH-like kinase 5; EPHB2; EPHB2_HUMAN; Ephrin type B receptor 2; Ephrin type-B receptor 2; EPHT 3; ERK; ETECK; hEK5; Nuk; Prkm 5; Receptor protein tyrosine kinase HEK 5; Renal carcinoma antigen NY-REN-47; Sek 3; Tyro 5; Tyrosine protein kinase receptor CEK 5; Tyrosine protein kinase receptor EPH 3; Tyrosine protein kinase receptor QEK 5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5;



Purified recombinant fragment of human EphB2 expressed in E. Coli.


Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

PTMs - P29323 As Substrate

Site PTM Type Enzyme
S148 Phosphorylation
T276 Phosphorylation
Y481 Phosphorylation
Y504 Phosphorylation
Y524 Phosphorylation
S575 Phosphorylation
Y577 Phosphorylation
T578 Phosphorylation
K580 Ubiquitination
Y584 Phosphorylation
T585 Phosphorylation
S586 Phosphorylation
T590 Phosphorylation
Y596 Phosphorylation P29323 (EPHB2)
T601 Phosphorylation
Y602 Phosphorylation P29323 (EPHB2)
K645 Ubiquitination
Y742 Phosphorylation
S756 Phosphorylation
S763 Phosphorylation
S768 Phosphorylation
T775 Phosphorylation
S776 Phosphorylation
T779 Phosphorylation
Y780 Phosphorylation
T781 Phosphorylation
Y800 Phosphorylation
S898 Phosphorylation
Y912 Phosphorylation
T959 Phosphorylation
K1020 Acetylation
K1024 Acetylation

PTMs - P29323 As Enzyme

Substrate Site Source
O94989 (ARHGEF15) Y353 Uniprot
P10301 (RRAS) Y66 Uniprot
P29323 (EPHB2) Y596 Uniprot
P29323-2 (EPHB2) Y602 Uniprot
P29323-3 (EPHB2) Y603 Uniprot
P29323-2 (EPHB2) T775 Uniprot
P29323-2 (EPHB2) S776 Uniprot
P29323-3 (EPHB2) T776 Uniprot
P29323-3 (EPHB2) S777 Uniprot
P52799 (EFNB2) Y304 Uniprot
Q13224 (GRIN2B) Y1474 Uniprot
Q92823 (NRCAM) Y1276 Uniprot

Research Backgrounds


Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.


Autophosphorylated; ligand binding stimulates autophosphorylation on tyrosine residues.

Polyubiquitinated; ligand binding stimulates ubiquitination.

Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

Subunit Structure:

Heterotetramer upon binding of the ligand (By similarity). The heterotetramer is composed of an ephrin dimer and a receptor dimer. Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain) (By similarity). Interacts with ARHGEF15; mediates ARHGEF15 phosphorylation, ubiquitination and degradation by the proteasome (By similarity). Interacts with AQP1; involved in endolymph production in the inner ear (By similarity). Interacts with SPSB1 and SPSB4. The phosphorylated form interacts with RASA1 (via SH2 domain 1) (By similarity). Interacts with EFNA5 (By similarity).


Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

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