ZC3H8 Antibody | Affinity Biosciences

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ZC3H8 Antibody - Western blot analysis of extracts from HepG2 cells, using ZC3H8 antibody.

Product:	ZC3H8 Antibody
Catalog:	DF4624
Description:	Rabbit polyclonal antibody to ZC3H8
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Dog
Mol.Wt.:	40 KD; 34kD(Calculated).
Uniprot:	Q8N5P1
RRID:	AB_2836988

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(85%), Dog(92%)

Clonality:

Polyclonal

Specificity:

ZC3H8 Antibody detects endogenous levels of total ZC3H8.

RRID:

AB_2836988
Cite Format: Affinity Biosciences Cat# DF4624, RRID:AB_2836988.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Fliz1; Zc3h8; ZC3H8_HUMAN; Zinc finger CCCH domain containing protein 8; Zinc finger CCCH domain-containing protein 8;

Immunogens

Immunogen:

Uniprot:

Q8N5P1(ZC3H8_HUMAN) >>Visit HPA database.

Gene(ID):

ZC3H8(84524)

Sequence:

Q8N5P1 ZC3H8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDFENLFSKPPNPALGKTATDSDERIDDEIDTEVEETQEEKIKLECEQIPKKFRHSAISPKSSLHRKSRSKDYDVYSDNDICSQESEDNFAKELQQYIQAREMANAAQPEESTKKEGVKDTPQAAKQKNKNLKAGHKNGKQKKMKRKWPGPGNKGSNALLRNSGSQEEDGKPKEKQQHLSQAFINQHTVERKGKQICKYFLERKCIKGDQCKFDHDAEIEKKKEMCKFYVQGYCTRGENCLYLHNEYPCKFYHTGTKCYQGEYCKFSHAPLTPETQELLAKVLDTEKKSCK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Rabbit

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N5P1 As Substrate

Q8N5P1

Site	PTM Type	Source
S22	Phosphorylation	Uniprot
T32	Phosphorylation	Uniprot
K43	Sumoylation	Uniprot
S56	Phosphorylation	Uniprot
S59	Phosphorylation	Uniprot
K61	Ubiquitination	Uniprot
S62	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
S68	Phosphorylation	Uniprot
S70	Phosphorylation	Uniprot
K71	Methylation	Uniprot
Y73	Phosphorylation	Uniprot
Y76	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
S86	Phosphorylation	Uniprot
T121	Phosphorylation	Uniprot
K140	Acetylation	Uniprot
K147	Acetylation	Uniprot
S165	Phosphorylation	Uniprot
K175	Ubiquitination	Uniprot
Y199	Phosphorylation	Uniprot
Y229	Phosphorylation	Uniprot

Research Backgrounds

Q8N5P1_ZC3H8_HUMAN

Function:

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Subcellular Location:

Nucleus.
Note: Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes.

Subunit Structure:

Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with ICE1 (via C-terminus domain).

Family&Domains:

The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ZC3H8 Antibody - #DF4624