IKZF2 Antibody - #DF4656
(2)
| Product: | IKZF2 Antibody |
| Catalog: | DF4656 |
| Description: | Rabbit polyclonal antibody to IKZF2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 56 KD; 60kD(Calculated). |
| Uniprot: | Q9UKS7 |
| RRID: | AB_2837007 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
IKZF2 Antibody detects endogenous levels of total IKZF2.
RRID:
AB_2837007
Cite Format: Affinity Biosciences Cat# DF4656, RRID:AB_2837007.
Cite Format: Affinity Biosciences Cat# DF4656, RRID:AB_2837007.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ANF1A2; HELIOS; IKAROS family zinc finger 2; Ikaros family zinc finger protein 2; IKZF 2; IKZF2; IKZF2_HUMAN; MGC34330; Zinc finger DNA binding protein Helios; Zinc finger protein Helios; Zinc finger protein subfamily 1A 2; ZNF1A2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9UKS7 IKZF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METEAIDGYITCDNELSPEREHSNMAIDLTSSTPNGQHASPSHMTSTNSVKLEMQSDEECDRKPLSREDEIRGHDEGSSLEEPLIESSEVADNRKVQELQGEGGIRLPNGKLKCDVCGMVCIGPNVLMVHKRSHTGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRSSLEEHKERCHNYLQNVSMEAAGQVMSHHVPPMEDCKEQEPIMDNNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Chicken
80
Zebrafish
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UKS7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T11 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K111 | Acetylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Sumoylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| Y355 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K442 | Sumoylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| T447 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| K459 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Associates with Ikaros at centromeric heterochromatin.
Subcellular Location:
Nucleus.
Subunit Structure:
Interacts with IKZF4 AND IKZF5.
Family&Domains:
Belongs to the Ikaros C2H2-type zinc-finger protein family.
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